Record details

Living Modified Organism identity

The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name

Roundup Ready™ sugar beet

Transformation event

H7-1

Unique identifier

KM-ØØØH71-4

Developer(s)

Record #14925

Monsanto

800 North Lindbergh Blvd.

St. Louis, MO

United States of America, 63167

Phone:	+ 1 314 694-1000
Fax:	+1 314 694-3080
Url:	Monsanto

Description

H7-1 sugar beet was modified with the insertion of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Agrobacterium tumefaciens, thereby conferring tolerance to the herbicide glyphosate.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #12097

Beta vulgaris - Common beet, Sugarbeet, BETMA

Related LMOs

Record #109070

Sugar beet modified for herbicide tolerance

Resistance to herbicides - Glyphosate

Characteristics of the transformation process

Vector

PV-BVGT08

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

FMV 35S promoter

#101507

0.67 Kb

Chloroplast transit peptide 2

#100365

0.23 Kb

5-enolpyruvylshikimate-3-phosphate synthase gene

#14979

1.37 Kb

rbcS-E9 gene terminator

#101877

0.64 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The nucleic acid sequence of the epsps gene was optimised for expression in plants.

LMO characteristics

Modified traits

Resistance to herbicides
- Glyphosate

Common use(s)

Food

Detection method(s)

External link(s)

KM-ØØØH71-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

KM-ØØØH71-4 - CropLife International Detection Methods Database

Additional Information

Both the endogenous EPSPS and the EPSPS introduced into the sugar beet plants by means of transformation catalyse the reaction of shikimate-3-phosphate with phosphoenolpyruvate to yield 5-enolpyruvylshikimate-3-phosphate, an intermediate stage in the biosynthesis of aromatic amino acids. In contrast to the endogenous EPSPS, the EPSPS inserted into the genetically modified sugar beet plants is not inhibited by glyphosate. The upstream position of the transit peptide CTP2 of the EPSPS derived from Arabidopsis thaliana causes the post-translational import of the chimeric protein into the chloroplasts.

Other relevant website address or attached documents

KM-ØØØH71-4 - OECD

KM-ØØØH71-4 - CERA GM Database

KM-ØØØH71-4 - ISAAA