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800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
|Phone:||+ 1 314 694-1000|
|Fax:||+1 314 694-3080|
Canola tolerant to the herbicide glyphosate produced by inserting
genes encoding the enzymes 5-enolypyruvylshikimate-3-phosphate
synthase (EPSPS) from the CP4 strain of Agrobacterium tumefaciens
and glyphosate oxidase (gox) from Ochrobactrum anthropi.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Brassica napus cv. Westar
- Agrobacterium-mediated DNA transfer
Chloroplast transit peptide 2
5-enolpyruvylshikimate-3-phosphate synthase gene
rbcS-E9 gene terminator
Glyphosate oxidoreductase gene
rbcS-E9 gene terminator
Southern blot analysis indicated that a single complete copy of the
T-DNA was integrated into the host genome. No segments of the
vector backbone were detected in the LMO.
The CP4 EPSPS gene codes for the enzyme
5-enolpyruvylshikimate-3-phosphate synthase that is present in all
plants, bacteria and fungi. The EPSPS gene put into GT200 was
isolated from strain CP4 of the common soil bacterium Agrobacterium
tumefaciens and is a glyphosate tolerant form of EPSPS. The EPSPS
enzyme is part of an important biochemical pathway in plants called
the shikimate pathway that is involved in the production of
aromatic amino acids and other aromatic compounds. When
conventional canola plants are treated with glyphosate, the plants
cannot produce the aromatic amino acids needed to grow and survive.
EPSPS is not present in mammals, birds or aquatic life forms, which
do not synthesize their own aromatic amino acids. For this reason,
glyphosate has little toxicity to these organisms. The EPSPS enzyme
is naturally present in foods derived from plant and microbial
The canola line GT200 contains a second gene that codes for a
modified version of glyphosate oxidase (GOX) enzyme that is
ubiquitous in nature. The goxv247 gene inserted into GT200 was
isolated from strain LBAA of the bacterium Ochrobactrum anthropi.
Glyphosate oxidase (GOX) enzyme accelerates the normal breakdown of
the herbicide glyphosate into two non-toxic compounds,
aminomethylphosphonic acid (AMPA) and glyoxylate. AMPA is the
principal breakdown product of glyphosate and is degraded by
several microorganisms, while glyoxylate is commonly found in plant
cells and is broken down by the glyoxylic pathway for lipid