NMK-89812-3 - New Leaf™ Russet Burbank potato | BCH-LMO-SCBD-14790 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 25 Apr 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
New Leaf™ Russet Burbank potato
EN
BT06 (RBBT06)
Yes
NMK-89812-3
Colorado potato beetle resistant potatoes produced by inserting the cry3A gene from Bacillus thuringiensis. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin is used as a selection marker.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: Russet Burbank
EN
  • NMK-89675-1 - New Leaf™ Russet Burbank potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • NMK-89167-6 - New Leaf™ Russet Burbank potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • NMK-89175-5 - New Leaf™ Russet Burbank potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • NMK-89593-9 - New Leaf™ Russet Burbank potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • NMK-896Ø1-8 - New Leaf™ Russet Burbank potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • NMK-899Ø6-7 - New Leaf™ Russet Burbank potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
Characteristics of the modification process
PV-STBT02
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The coding sequence of the Cry3A gene was modified to plant preferred codons. This resulted in changes to 399 of 1791 nucleotides but there were no changes to the resulting amino acid sequence.

Southern blot analysis indicated that a single insert of the transformation cassette was integrated into the host genome with no detectable rearrangements, insertions or segments of the vector backbone.
EN
LMO characteristics
EN
  • Food
Detection method(s)
EN
Additional Information
Cry proteins, of which Cry3A is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow causing gut paralysis and eventual death due to bacterial sepsis. Cry3A is insecticidal only when eaten by the larvae of coleopteran insects such as Colorado potato beetle and its specificity of action is directly attributable to the presence of specific binding sites in the target insects.
EN
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