SYN-BTØ11-1 - YieldGard™ maize | BCH-LMO-SCBD-14797 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 08 May 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
YieldGard™ maize
Bt 11 (X4334CBR, X4734CBR)
Insect-resistant and herbicide tolerant maize produced by inserting the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces viridochromogenes to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • Bt-10 Maize
    | Resistance to antibiotics (Ampicillin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate)
Characteristics of the modification process
pZO1502 Derived from pUC18
  • Direct DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-103625-3 Alcohol dehydrogenase 1, intron 6 | Zea mays (Maize, Corn, MAIZE)
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    BCH-GENE-SCBD-103867-2 Alcohol dehydrogenase 1, intron 2 | Zea mays (Maize, Corn, MAIZE)
Maize line Bt11 was genetically modified to contain two novel genes, cry1Ab and pat, for insect and herbicide tolerance respectively. Both genes were introduced into a maize line by particle acceleration (biolistic) transformation.

Information on the inserted DNA sequences:
- 35S promoters derived from cauliflower mosaic virus (CaMV) and 35S-1 originated from the CM1841 isolate of CaMV as a 500 Ddel to Ddel fragment, subsequently converted to Sacl sites and 35S-2 originated from the Cabb-S strain of CaMV as a n Alul to Ddel fragments (ca 425bp), whose ends were subsequently modified.
- introns derived from the maize alcohol dehydrogenase 1S gene and were used to enhance heterologous gene expression.
- Btk gene which is an altered version of the full length cry1A(b) gene of Bacillus thuringiensis var kurstaki HD-1. The Btk was obtained as a 1.8kb Nco-Bg/ll fragment. The truncated Btk protein is identical to the N-terminal 615 amino acids of the native Btk protein of 1155 amino acids.
- pat gene (phosphinotricin acetyl transferase) cloned from the soil microorganism, Streptomyces viridochromogenes strain Tu494.  Alteration did not result in any amino acid sequence changes.
- nos terminator consisting of 423-678 of the nopaline synthase gene of Agrobacterium tumefaciens plus added restriction sites.

Vector information
Plasmid pZO1502 is the vector used for the transformation of Bt maize.  This is a derivative of plasmid pUC18. The plasmid has a molecular weight of 2.7 kb and contains the following sequences:
- the prokaryotic gene bla (also called ampR) under a procaryotic promoter encoding β-lactamase, which confers resistance to ampicillin; it is used as a bacterial selectable marker; 
- the gene lac Z, encoding a portion of a β-galactosidase, this gene is not functional;
- the pUC origin of replication derived from the plasmid pBR 322 carrying a mutation.
LMO characteristics
  • Food
  • Feed
Additional Information