Herculex™ I Roundup Ready™ 2 maize

This LMO is the result of a cross between LMOs DAS-Ø15Ø7-1 and MON-ØØ6Ø3-6.

The MON-ØØ6Ø3-6 line of maize was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. NK603 contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate.

Corn line DAS-Ø15Ø7-1 is modified with the insertion of the Cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

PHP8999 and PV-ZMGT32

Notes about the Other(s) sequence(s) specific to this LMO

DNA construct from TC1507:

P-ubiZM1 (1.98) >> cry1F (1.82) >> T-ORF25 (0.72)* | P-35S (0.55) >> pat (0.55) >> T-35s (0.2)

DNA construct from NK603:

P-Ract1 >> I-Ract1 >> TS-CTP2 >> cp4-epsps >> T-nos | spacer (217) |  P-e35S >> I-Hsp70  >> TS-CTP2 >> cp4-epsps >> T-nos

Note: the symbols >> and << indicate the sense and anti-sense direction of transcription, respectively, between different elements (typically, promoter - coding sequence - terminator). If present, the symbol | indicates elements that may be cloned adjacently in a cassette but that presumably are not transcribed together. The numbers in parenthesis indicate the approximate length in Kb of each genetic element.

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* The EFSA document attached below indicates that the 3' sequence from the Agrobacterium tumefaciens mannopine synthase gene was used as terminator of the cry1F gene.