FLO-ØØØ66-9 - Long vase life carnation | BCH-LMO-SCBD-14839 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 26 Jan 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Long vase life carnation
EN
66
Yes
FLO-ØØØ66-9
Long vase life (delayed senescence) carnations produced by inserting a truncated copy of the carnation 1-amino-cyclopropane-1-carboxylic acid (ACC) synthase encoding gene in order to suppress expression of the endogenous unmodified gene, which is required for normal ethylene biosynthesis. Tolerance to sulfonyl urea herbicides was via the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from tobacco.

NOTE: This LMO was formerly referred to with the UID FLO-ØØØ66-8.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: Ashley
EN
Characteristics of the modification process
pWTT2160
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )
    Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
  • BCH-GENE-SCBD-15012-5 1-amino-cyclopropane -1-carboxylic acid synthase gene | Dianthus caryophyllus (Carnation, DIACA)
    Protein coding sequence | Changes in physiology and/or production (Ripening)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )
    Terminator
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
The enzyme 1-aminocyclopropane-1-carboxyllic acid (ACC) synthase, normally found in carnations, is responsible for the conversion of s-adenosylmethionine to ACC, which is the immediate precursor of ethylene. The transgenic carnation line 66 was developed using recombinant DNA techniques to display suppressed ACC synthase activity, and thus reduced ethylene synthesis and therefore longer vase life, by inserting an additional sense orientation, truncated copy of the ACC synthase encoding gene. The presence of the truncated ACC synthase gene suppresses the normal expression of the native ACC synthase gene, and while not completely understood, the mechanism of “downregulation” is likely linked to the coordinate suppression of transcription of both the endogenous gene and the introduced truncated ACC synthase gene.
EN
LMO characteristics
EN
  • Ornamental
Detection method(s)
EN
Additional Information
EN
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