The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
|Phone:||+ 1 314 694-1000|
|Fax:||+1 314 694-3080|
Cotton line 1445 was genetically engineered to express resistance
to glyphosate, the active ingredient in the herbicide Roundup®,
allowing for its use as a weed control option. In order to obtain
field tolerance to glyphosate herbicide, a bacterial gene encoding
a glyphosate-tolerant form of the enzyme
5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was introduced
into the cotton genome by Agrobacterium-mediated
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
- Agrobacterium-mediated DNA transfer
Chloroplast transit peptide 2
5-enolpyruvylshikimate-3-phosphate synthase gene
rbcS-E9 gene terminator
Neomycin Phosphotransferase II
Nopaline Synthase Gene Terminator
Information on the inserted DNA sequences
Molecular characterization demonstrates that one T-DNA insert was
integrated into the cotton genome to produce cotton 1445. The
insert contains the CoMV promoter region, a codon optimised
cp4epsps coding sequence, nptII, aad (that is not expressed in
plants) and a portion of ori-V coding regions.
The plasmid vector PV-GHGT07 contains well characterized DNA
segments required for selection and replication of the plasmid in
the bacteria as well as a right border for initiating the region of
T-DNA, into the plant genomic DNA. The plant expression
vectors were assembled, transformed in E. coli and mated into the
ABI Agrobacterium strain by the triparental conjugation system
using the helper plasmid pRK2013. The binary ABI strain contains
the disarmed (lacking the T-DNA phytohormone genes) pTiC58 plasmid
pMP90RK in a choramphenicol-resistant derivative of the A.
tumefaciens strain A208. The disarmed pMP90RK Ti plasmid does not
carry the T-DNA phytohormone genes and is unable to grow the crown
- Resistance to antibiotics
- Resistance to herbicides
Coding sequences of the EPSPS gene was altered for optimal
expression in plants.
The EPSPS enzyme is present in all plants, bacteria, and fungi, and
is part of an important biochemical pathway called the shikimate
pathway, which is involved in the production of aromatic amino
acids and other aromatic compounds. When conventional cotton plants
are treated with glyphosate, the plants cannot produce the aromatic
amino acids needed to grow and survive. EPSPS is not present in
mammals, birds or aquatic life forms, which do not synthesize their
own aromatic amino acids. For this reason, glyphosate has little
toxicity to these organisms. The EPSPS enzyme is naturally present
in foods derived from plant and microbial sources.