MON-ØØ531-6 × MON-Ø1445-2 - Roundup Ready™ Bollgard™ Cotton | BCH-LMO-SCBD-14883 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 24 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ Bollgard™ Cotton
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1445 x 531 (MON531 X MON1445)
Yes
MON-ØØ531-6 × MON-Ø1445-2
The stacked cotton line MON531 X MON1445 expresses two novel proteins: the delta-endotoxin Cry1Ac which confers resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm, and the CP4 EPSPS protein which confers tolerance to the herbicide glyphosate. The insecticidal protein is produced by the cry1Ac gene from MON531, and the CP4 EPSPS protein is from the cp4 epsps gene from MON1445. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new cotton line.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)
    Crops
  • BCH-LMO-SCBD-14775-16 Living Modified Organism MON-ØØ531-6 - Bollgard™ cotton
    Monsanto | Resistance to antibiotics (Kanamycin, Streptomycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-LMO-SCBD-14880-14 Living Modified Organism MON-Ø1445-2 - Roundup Ready™ cotton
    Monsanto | Resistance to antibiotics (Kanamycin, Streptomycin), Resistance to herbicides (Glyphosate)
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Characteristics of the modification process
PV-GHBK04 and PV-GHGT07
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Streptomycin)
  • BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
    Promoter
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-103856-6 α' subunit of β-conglycinin gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Terminator
A stacked insect-resistant and glyphosate-tolerant cotton derived from conventional cross-breeding of MON-ØØ531-6 and MON-Ø1445-2.  Tolerance to the herbicide glyphosate produced through incorporation of the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase (epsps), that confers tolerance to the herbicide glyphosate and resistance to lepidoptera insects from the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin.

DNA insert from vector PV-GHBK04
From the MON531 parental organism. Contains coding sequences and regulatory elements for cry1A(c) and nptII. The aadA gene was also integrated into the host genome, however it is only expressed in bacterial cells.

DNA insert from vector PV-GHGT07
From the MON1445 parental organism. contains coding sequences and regulatory elements for epsps and nptII. The aadA gene was also integrated into the host genome, however it is only expressed in bacterial cells. The PV-GHGT07 vector also contained a coding sequence for the Glyphosate oxidoreductase (GOX) gene however southern blot analysis indicated that that it was not integrated into the host genome.

For additional information on this LMO, please refer to the records of the parental LMOs.
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LMO characteristics
EN
  • Food
  • Feed
  • Fiber/textile
Additional Information
EN
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