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The cotton line 3006-210-23 was genetically engineered to resist
attack from lepidopteran insect pests such as the cotton bollworm,
pink bollworm, tobacco budworm, beet armyworm, and soybean looper.
This insect resistance is conferred by the cry1Ac gene, originally
isolated from the common soil bacterium Bacillus thuringiensis (Bt)
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Line 'Germain's Acala GC510'
- Agrobacterium-mediated DNA transfer
Phosphinothricin N-acetyltransferase gene
ORF25 PolyA Terminator sequence
Ubiquitin gene promoter
Coding sequences of Cry1A(c) and pat genes altered for optimal
expression in plant cells.
Southern blot analysis demonstrated that event 3006-210-23 contains
a single integration of the transgenic DNA, including one intact
copy of the cry1Ac gene, one intact copy of the pat gene, and
single, intact copies of each of the regulatory elements. Analysis
also indicated that there was no integration of segments from the
erythromycin resistance gene.
- Resistance to diseases and pests
- Lepidoptera (butterflies and moths)
The cry1Ac gene produces the insect control protein Cry1Ac, a
delta-endotoxin, in the plant tissues. Cry proteins, of which
Cry1Ac is only one, act by selectively binding to specific sites
localized on the lining of the midgut of susceptible insect
species. Following binding, pores are formed that disrupt midgut
ion flow, causing gut paralysis and eventual death due to bacterial
sepsis. Cry1Ac is insecticidal only when eaten by the larvae of
lepidopteran insects (moths and butterflies), and its specificity
of action is directly attributable to the presence of specific
binding sites in the target insects. There are no binding sites for
delta-endotoxins of B. thuringiensis on the surface of mammalian
intestinal cells, therefore, livestock animals and humans are not
susceptible to these proteins.