BXN-1Ø211-9 - BXN™ cotton | BCH-LMO-SCBD-14941 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 08 Jun 2006 last updated: 08 Apr 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
BXN™ cotton
EN
10211
Yes
BXN-1Ø211-9
Cotton tolerant to oxynil herbicides, through introduction of the bxn gene isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae which codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds.  The aphII gene was isolated from the bacterium Eschericia coli confers tolerance to the antibiotic kanamycin (used as a selectable marker).
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Line: Coker 315
EN
  • BXN-1Ø215-4 - BXN™ cotton
    | Resistance to antibiotics (Kanamycin), Resistance to herbicides (Bromoxynil)
  • BXN-1Ø224-4 - BXN™ cotton
    | Resistance to antibiotics (Kanamycin), Resistance to herbicides (Bromoxynil)
  • BXN-1Ø222-2 - BXN™ cotton
    | Resistance to antibiotics (Kanamycin), Resistance to herbicides (Bromoxynil)
Characteristics of the modification process
pBrx75
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
EN
LMO characteristics
EN
  • Food
  • Fiber/textile
Detection method(s)
EN
Additional Information
The BXN™ cotton line was genetically engineered to express tolerance to oxynil herbicides, including bromoxynil and ioxynil. Oxynil herbicides act by blocking electron flow during the light reaction of photosynthesis, inhibiting cellular respiration in dicotyledonous plants. Oxynil herbicides applied at rates recommended for effective weed control are toxic to conventional cotton varieties. The modified cotton line BXN™ contains the bxn gene for oxynil tolerance, and allows farmers to use oxynil herbicides for weed control in the cultivation of cotton.

The bxn gene was isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae and codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds. The nitrilase encoding gene was introduced into the cotton genome using Agrobacterium-mediated transformation, and the bacterial form of the enzyme expressed in this transgenic cotton line functions the same as that found in monocot plants such as corn, wheat and barley.

The kanamycin-resistance gene (aphII), isolated from the bacterium Escherichia coli codes for an enzyme (aminoglycoside 3'-phosphotransferase II) that phosphorylates kanamycin , thereby preventing it from binding to ribosomes and rendering the cells resistant. This protein is ubiquitous in the environment and it degrades rapidly in vitro in simulated mammalian gastric and intestinal fluids.
EN
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