BPS-25271-9 - Amflora™Potato | BCH-LMO-SCBD-15100 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 19 Jun 2006 last updated: 13 May 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Amflora™Potato was modified to produce less amylose starch and thus a higher content of amylopectin. This was done through anti-sense suppression of the granule bound starch synthase (GBSS) protein.

GBSS enzyme is one of the key enzymes in the biosynthesis of starch and catalyses the formation of amylose. When the gene is inactivated through antisense technology, the starch produced has little or no amylose and consists of branched amylopectin, which modifies the physical properties of the starch and is advantageous for the starch processing industry.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)
  • BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | altered carbohydrate composition: increased amylopectin content
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
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    BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
The Granule-bound starch synthase gene was inserted in an anti-sense orientation, relative to the promoter therefore resulting in the suppression of endogenous GBSS.

Southern blot analysis indicated that a single copy of the insert was integrated into the host genome with no integration of fragments of the vector backbone.
LMO characteristics
  • Other (Industrial)
Additional Information