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Living Modified Organism (LMO)
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YieldGard™ VT™ Rootworm/RR2™ Maize
EN
MON88017
MON-88Ø17-3
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OrganizationMonsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, United States of AmericaPhone: + 1 314 694-1000,Fax: +1 314 694-3080,Email:Website: http://www.monsanto.com,
Corn rootworm-resistant maize line MON88017 was produced using recombinant-DNA techniques to express the cry3Bb1 gene encoding a Coleopteran-specific insecticidal protein from Bacillus thuringiensis (subsp. kumamotoensis) in order to control infestation with corn root worm, and the cp4 epsps gene from the soil bacterium Agrobacterium ssp. strain CP4.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
PV-ZMIR39
EN
- Agrobacterium-mediated DNA transfer
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0.933 kb
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0.460 kb
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0.227 kb
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1.367 kb
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0.255 kb
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0.612 kb
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0.070 kb
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0.460 kb
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1.961 kb
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0.233 kb
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Some of these genetic elements may be present as fragments or truncated
forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase geneProtein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-14993-5 Cry3Bb1 | Bacillus thuringiensis - Bt, Bacillus, BACTUProtein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | (Rice)Promoter
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | (Rice)Intron
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | (Thale cress)Transit signal
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene TerminatorTerminator
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoterPromoter
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BCH-GENE-SCBD-100354-6 5' untranslated leader from chlorophyll a/b-binding protein | (Wheat)Leader sequence
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BCH-GENE-SCBD-100356-6 Heat shock protein 17.3 terminator | (Wheat)Terminator
The wild-type cry3Bb1 coding sequence was modified to encode six specific amino acid substitutions, resulting in the synthetic cry3Bb1 coding sequence present in the vector. The differences at the six positions are: 2A (insertion), H232R, S312L, N314T, E318K, Q349R.
Molecular analyses of MON 88017 confirmed that single copies of the cp4 epsps and cry3Bb1 genes are integrated at a single locus in the corn genome with all expression elements intact and no plasmid bacterial backbone present. Plasmid PV-ZMIR39 contains the left and right transfer DNA (T-DNA) border sequences that facilitate transformation.
Molecular analyses of MON 88017 confirmed that single copies of the cp4 epsps and cry3Bb1 genes are integrated at a single locus in the corn genome with all expression elements intact and no plasmid bacterial backbone present. Plasmid PV-ZMIR39 contains the left and right transfer DNA (T-DNA) border sequences that facilitate transformation.
- Food
- Feed
- MON-88Ø17-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-88Ø17-3 - CropLife International Detection Methods Database [ English ]
- MON-88Ø17-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-88Ø17-3 - CropLife International Detection Methods Database ( CropLife ) [ English ]
The cry3Bb1 gene encodes the insect control protein Cry3Bb1, a delta-endotoxin. The toxin protects the plant from the Coleopteran insects western corn rootworm (Diabrotica virgifera), northern corn rootworm (D. barberi), and Mexican corn rootworm (D. virgifera zeae). Cry proteins, of which Cry3Bb1 is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry3Bb1 is lethal only when eaten by Coleopteran species, including corn root worm, and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for the delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
The cp4 epsps gene encodes for a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is highly tolerant to inhibition by glyphosate.
The cp4 epsps gene encodes for a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is highly tolerant to inhibition by glyphosate.
- MON88017 - GMO Compass [ English ]
- MON-88O17-3 - CERA [ English ]
- MON-88Ø17-3 - Monsanto.pdf [ English ]
- MON-88Ø17-3 - OECD [ English ]
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