MON-88Ø17-3 - YieldGard™ VT™ Rootworm/RR2™ Maize | BCH-LMO-SCBD-15106 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 19 Jun 2006 last updated: 16 May 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
YieldGard™ VT™ Rootworm/RR2™ Maize
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MON88017
MON-88Ø17-3
  • - Organization: Monsanto () | BCH-CON-SCBD-14925-3
    Organization
    Monsanto ()
    800 North Lindbergh Blvd.
    St. Louis, MO
    63167, United States of America
    Phone: + 1 314 694-1000,
    Fax: +1 314 694-3080,
    Email:
Corn rootworm-resistant maize line MON88017 was produced using recombinant-DNA techniques to express the cry3Bb1 gene encoding a Coleopteran-specific insecticidal protein from Bacillus thuringiensis (subsp. kumamotoensis) in order to control infestation with corn root worm, and the cp4 epsps gene from the soil bacterium Agrobacterium ssp. strain CP4.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
PV-ZMIR39
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The wild-type cry3Bb1 coding sequence was modified to encode six specific amino acid substitutions, resulting in the synthetic cry3Bb1 coding sequence present in the vector. The differences at the six positions are: 2A (insertion), H232R, S312L, N314T, E318K, Q349R.

Molecular analyses of MON 88017 confirmed that single copies of the cp4 epsps and cry3Bb1 genes are integrated at a single locus in the corn genome with all expression elements intact and no plasmid bacterial backbone present. Plasmid PV-ZMIR39 contains the left and right transfer DNA (T-DNA) border sequences that facilitate transformation.
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LMO characteristics
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  • Food
  • Feed
Additional Information
The cry3Bb1 gene encodes the insect control protein Cry3Bb1, a delta-endotoxin. The toxin protects the plant from the Coleopteran insects western corn rootworm (Diabrotica virgifera), northern corn rootworm (D. barberi), and Mexican corn rootworm (D. virgifera zeae).  Cry proteins, of which Cry3Bb1 is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry3Bb1 is lethal only when eaten by Coleopteran species, including corn root worm, and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for the delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

The cp4 epsps gene encodes for a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is highly tolerant to inhibition by glyphosate.
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 39
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 25
Country's Decision or any other Communication Living modified organism(s) 32
Living Modified Organism Recipient Organism” or “Parental Organisms 25
Living Modified Organism Related LMO(s) 1
Risk Assessment generated by an independent or non-regulatory process Living modified organism(s) 1