The cry34Ab1 and cry35Ab1 were cloned from Bacillus thuringiensis
strain PS149B1 and the coding sequence of both genes has been
adapted to the codon usage in maize as to achieve expression in the
Sequence analysis of 59122 done by the European Food Safety
Authority indicated that this LMO contains one complete copy of the
T-DNA of PHP17662 without internal rearrangements. All three gene
cassettes, cry34Ab1, cry35Ab1 and pat, are intact within the
transgenic event. The DNA sequences of the genes in 59122 are
identical to those in the original plasmid except for two
nucleotide differences in the wheat peroxidise promoter. At the 5'
T-DNA end a deletion of 22 bp is observed and at the 3' T-DNA end a
deletion of 25 bp is observed. The absence of vector backbone in
maize 59122 was also demonstrated.