DAS-59122-7 - Herculex™ RW Rootworm Protection maize | BCH-LMO-SCBD-15165 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 20 Jun 2006 last updated: 15 Jan 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herculex™ RW Rootworm Protection maize
The transgenic maize line DAS-59122-7 was genetically modified to resist Coleopteran insects (e.g. western corn rootworm, northern corn rootworm and Mexican corn rootworm) and to tolerate herbicides containing glufosinate ammonium.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • DAS-59132-8 - Insect resistant Herbicide tolerant Maize
    | Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Coleoptera (beetles)), Resistance to herbicides (Glufosinate)
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The cry34Ab1 and cry35Ab1 were cloned from Bacillus thuringiensis strain PS149B1 and the coding sequence of both genes has been adapted to the codon usage in maize as to achieve expression in the maize plant.

Sequence analysis of 59122 done by the European Food Safety Authority indicated that this LMO contains one complete copy of the T-DNA of PHP17662 without internal rearrangements. All three gene cassettes, cry34Ab1, cry35Ab1 and pat, are intact within the transgenic event. The DNA sequences of the genes in 59122 are identical to those in the original plasmid except for two nucleotide differences in the wheat peroxidise promoter. At the 5’ T-DNA end a deletion of 22 bp is observed and at the 3’ T-DNA end a deletion of 25 bp is observed. The absence of vector backbone in maize 59122 was also demonstrated.
LMO characteristics
  • Food
  • Feed
  • Biofuel
Additional Information
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