Maize line DAS-59122 x TC1507 x NK603 contains the cryIF gene from Bacillus thuringiensis subsp. kurstaki, which codes for a Bt-toxin, which protects the plant against the european corn borer (Ostrinia nubilalis) and other lepidopterans such as the pink borer (Sesamia spp.), fall armyworm (Spodoptera frugiperda), black cutworm (Agrotis ipsilon) and southwestern corn borer (Diatraea grandiosella), as well as cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis which protect the plant from the Coleopteran insects western corn rootworm (Diabrotica virgifera), northern corn rootworm (D. barberi), and Mexican corn rootworm (D. virgifera zeae).

This line also contains stacked tolerance to glufosinate ammonium and glyphosate containing herbicides, through incorporation of the cp4 epsps gene from the soil bacterium Agrobacterium ssp. strain CP4, which encodes for a version of EPSPS that is highly tolerant to inhibition by glyphosate,  and the pat gene from the soil bacterium Streptomyces viridochromogenes, which codes for the enzyme Phosphinothricin-Acetyltransferase (PAT) and leads to increased tolerance to glufosinate-containing herbicides.