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800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
|Phone:||+ 1 314 694-1000|
|Fax:||+1 314 694-3080|
Squash resistant to infection by Cucumber mosaic cucumovirus (CMV),
Zucchini yellow mosaic potyvirus (ZYMV) and Watermelon mosaic
potyvirus 2 (WMV-2) through incorporation of virus-derived
sequences that encode the coat proteins (CPs) from each of these
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
SEM-ØZW2Ø-7 - Virus-resistant squash
Resistance to antibiotics - Kanamycin
Resistance to diseases and pests - Viruses - Mosaic virus - Watermelon mosaic virus-2 (WMV2), Zucchini yellow mosaic virus (ZYMV)
- Agrobacterium-mediated DNA transfer
Ti plasmid left border repeat
Neomycin Phosphotransferase II
WMV-2 coat protein
Ti plasmid right border repeat
All three coat protein genes were fused to the 5' untranslated
sequence from CMV to enhance translation of the transgene mRNA.
- Resistance to antibiotics
- Resistance to diseases and pests
- Mosaic virus
- Cucumber mosaic virus (CMV)
- Watermelon mosaic virus-2 (WMV2)
- Zucchini yellow mosaic virus (ZYMV)
Southern blot analysis of genomic DNA from CZW-3 indicated the
presence of a single copy of the T-DNA containing intact sequences
corresponding to the CP encoding genes form CMV, ZYMV, and WMV2,
integrated at a single site. Additional analyses verified the
presence of a single copy of the NPTII encoding neo antibiotic
resistance marker gene, and no incorporation of plasmid backbone
sequences outside of the T-DNA region.
The CZW-3 squash line was developed using recombinant DNA
techniques to resist infection by CMV, ZYMV, and WMV2 by inserting
virus-derived sequences that encode the coat proteins (CPs) from
each of these viruses. The introduced viral sequences do not result
in the formation of any infectious particles, nor does their
expression result in any disease pathology.
This transgenic squash exhibits "pathogen-derived resistance" to
infection and subsequent disease caused by CMV, ZYMV, and WMV2
through a process that is related to viral cross-protection.
Although the exact mechanism by which the viral protection occurs
is unknown, most evidence suggests that expression of viral CP by a
plant interferes with one of the first steps in viral replication,
uncoating (removal of CP) from the incoming virus. Other modes of
action of cross-protection have also been suggested.