SEM-ØZW2Ø-7 - Virus-resistant squash | BCH-LMO-SCBD-15218 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 23 Jun 2006 last updated: 06 Dec 2012
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Virus-resistant squash
EN
ZW20
Yes
SEM-ØZW2Ø-7
Squash resistant to infection by Zucchini yellow mosaic potyvirus (ZYMV) and Watermelon mosaic potyvirus 2 (WMV-2) through incorporation of virus-derived sequences that encode the coat proteins (CPs) from both of these viruses.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • SEM-ØCZW3-2 - Virus-resistant squash
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Viruses, Mosaic virus, Cucumber mosaic virus (CMV), Watermelon mosaic virus-2 (WMV2), Zucchini yellow mosaic virus (ZYMV))
Characteristics of the modification process
ZYMV72/WMBN22
EN
  • Agrobacterium-mediated DNA transfer
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15025-3 ZYMV coat protein | Zucchini yellow mosaic virus (ZYMV)
    Protein coding sequence | Resistance to diseases and pests (Viruses, Mosaic virus, Zucchini yellow mosaic virus (ZYMV))
  • BCH-GENE-SCBD-15024-4 WMV-2 coat protein | Watermelon mosaic virus
    Protein coding sequence | Resistance to diseases and pests (Viruses, Mosaic virus, Watermelon mosaic virus-2 (WMV2))
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
The WMV2 CP gene was fused to the 5' intergenic region and the first 48 nucleotides (N-terminus) of the CMV coat protein gene to enhance translation of the transgene mRNA.

The ZYMV CP coding sequence was fused to the CMV 5' untranslated region to enhance translation.

While the gene encoding neomycin phosphotransferase (NPTII) was incorporated into the Ti plasmid vector used for transformation, only plants lacking this gene were selected for commercialization. The gene encoding NPTII is, therefore, not present in the ZW-20 genome. There was no incorporation of plasmid DNA sequences outside of the T-DNA region as verified by Southern blot analysis.
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LMO characteristics
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  • Food
Detection method(s)
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Additional Information
The ZW20 squash line was developed using recombinant DNA techniques to resist infection by ZYMV and WMV2 by inserting virus-derived sequences that encode the coat proteins (CPs) from each of these viruses. The introduced viral sequences do not result in the formation of any infectious particles, nor does their expression result in any disease pathology.

This transgenic squash exhibits “pathogen-derived resistance” to infection and subsequent disease caused by ZYMV and WMV2 through a process that is related to viral cross-protection. Although the exact mechanism by which the viral protection occurs is unknown, most evidence suggests that expression of viral CP by a plant interferes with one of the first steps in viral replication, uncoating (removal of CP) from the incoming virus. Other modes of action of cross-protection have also been suggested.
EN
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