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800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
|Phone:||+ 1 314 694-1000|
|Fax:||+1 314 694-3080|
Squash resistant to infection by Zucchini yellow mosaic potyvirus
(ZYMV) and Watermelon mosaic potyvirus 2 (WMV-2) through
incorporation of virus-derived sequences that encode the coat
proteins (CPs) from both of these viruses.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
SEM-ØCZW3-2 - Virus-resistant squash
Resistance to antibiotics - Kanamycin
Resistance to diseases and pests - Viruses - Mosaic virus - Cucumber mosaic virus (CMV), Watermelon mosaic virus-2 (WMV2), Zucchini yellow mosaic virus (ZYMV) Show detection method(s)
- Agrobacterium-mediated DNA transfer
WMV-2 coat protein
Neomycin Phosphotransferase II
The WMV2 CP gene was fused to the 5' intergenic region and the
first 48 nucleotides (N-terminus) of the CMV coat protein gene to
enhance translation of the transgene mRNA.
The ZYMV CP coding sequence was fused to the CMV 5' untranslated
region to enhance translation.
While the gene encoding neomycin phosphotransferase (NPTII) was
incorporated into the Ti plasmid vector used for transformation,
only plants lacking this gene were selected for commercialization.
The gene encoding NPTII is, therefore, not present in the ZW-20
genome. There was no incorporation of plasmid DNA sequences outside
of the T-DNA region as verified by Southern blot analysis.
- Resistance to antibiotics
- Resistance to diseases and pests
- Mosaic virus
- Watermelon mosaic virus-2 (WMV2)
- Zucchini yellow mosaic virus (ZYMV)
The ZW20 squash line was developed using recombinant DNA techniques
to resist infection by ZYMV and WMV2 by inserting virus-derived
sequences that encode the coat proteins (CPs) from each of these
viruses. The introduced viral sequences do not result in the
formation of any infectious particles, nor does their expression
result in any disease pathology.
This transgenic squash exhibits "pathogen-derived resistance" to
infection and subsequent disease caused by ZYMV and WMV2 through a
process that is related to viral cross-protection. Although the
exact mechanism by which the viral protection occurs is unknown,
most evidence suggests that expression of viral CP by a plant
interferes with one of the first steps in viral replication,
uncoating (removal of CP) from the incoming virus. Other modes of
action of cross-protection have also been suggested.