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800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
|Phone:||+ 1 314 694-1000
|Fax:||+1 314 694-3080
Medicago sativa, also known as alfalfa or lucerne, is a
perennial herbaceous legume. It contains the
5-enolpyruvylshikimate-3-phosphate synthase gene from
Agrobacterium tumefaciens strain CP4 to confer tolerance
to glyphosate, the active ingredient in the herbicide Roundup®, and
allow producers to use the herbicide a weed control option. Due to
the inclusion of the Figwort Mosaic Virus 356S promoter, herbicide
tolerance is constitutive and allows for weed control at anytime
(early, pre-plant, pre-emergence, and post-emergence).
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Proprietary alfalfa clone 'R2336.'
- Agrobacterium-mediated DNA transfer
HSP 70 5' untranslated leader sequence
Chloroplast transit peptide 2
5-enolpyruvylshikimate-3-phosphate synthase gene
rbcS-E9 gene terminator
Transcription of the herbicide tolerance gene,
5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps)
from Agrobacterium tumefaciens, is under control of the
Figwort Mosaic Virus 35S promoter and the Pisum sativum
Rubisco small subunit terminator. The transcript also contains the
Petunia hybrid heat shock protein 70 5'untranslated region
for enhanced translation and the Arabidopsis thaliana
chloroplast transit peptide 2 for targeting the translated
polypeptide to the chloroplast. Transcript level are expected to be
elevated due to the constitutive, viral promoter and the protein
sequestered in the chloroplasts.
- A single T-DNA integration site was detected by Southern blot
- The vector backbone was not integrated into the J163 genome
- A single basepair change (A to C) was detected in the J163
insertion. However, the change occurs in an intervening sequence
and thus is not expected to change the sequences transcribed or
Lucerne is a forage crop for feeding to dairy cattle and horses, as
well as a valuable feed crop for beef cattle, sheep and other
livestock. It is a nitrogen-fixing crop due to its symbiosis with
Rhizobium meliloti and has the ability to improve soil
The EPSPS gene is present in animals, fungi, bacteria and plants
and is responsible for aromatic amino acid biosynthesis.
Glycophosate binds to the plant version of the EPSPS gene and
blocks enzymatic biosynthesis of the aromatic amino acids and leads
to the death of the plant. The introduced gene was isolated from
the common soil bacterium Agrobacterium tumefaciens strain
CP4 and the form of EPSPS enzyme produced by this gene has a low
affinity for glycophosate, thus allowing for the continued
biosynthesis of aromatic amino acids.