ACS-ZMØØ3-2 × MON-ØØ81Ø-6 - Liberty Link™ Yieldgard™ maize | BCH-LMO-SCBD-15373 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 12 Jul 2006 last updated: 24 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Liberty Link™ Yieldgard™ maize
T25 x MON810
The stacked maize line ACS-ZMØØ3-2 x MON-ØØ81Ø-6 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of PAT and Cry1Ab genes. The expression of these genes are expected to confer resistance to Lepidoptera, and tolerant to glufosinate herbicide.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
  • BCH-LMO-SCBD-14767-14 Living Modified Organism ACS-ZMØØ3-2 - Liberty Link™ maize
    Bayer CropScience | Resistance to antibiotics (Ampicillin), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-14750-19 Living Modified Organism MON-ØØ81Ø-6 - YieldGard™ maize
    Monsanto | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis))
Characteristics of the modification process
pDH51, PV-ZMBK07 and PV-ZMGT10
  • Cross breeding
0.610 kb
0.800 kb
3.460 kb
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from ACS-ZMØØ3-2 vector pDH51
Glufosinate tolerance in T25 maize due to the enzyme phosphinothricin-N-acetyltransferase (PAT). The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound. Molecular analyses shows that it has a truncated copy of the bla gene (25% of the 5' end of the bla gene is missing in T25) and an intact Ori-pUC.

DNA insert from MON-ØØ81Ø-6 vector PV-ZMBK07 and PV-ZMGT10
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. This confers resistance to Lepidoptera pests. Two constructs PV-ZMBK07 and PV-ZMGT10 were used for transformation, but molecular analyses showed no elements from the PV-ZMGT10 construct were integrated and only the elements from construct PV-ZMBK07 have been integrated into its genome. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
  • Food
  • Feed
Additional Information