Record details

Living Modified Organism identity

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LMO name

Liberty Link™ Yieldgard™ maize

Transformation event

T25 x MON810

Unique identifier

ACS-ZMØØ3-2 x MON-ØØ81Ø-6

Developer(s)

Record #14925

Monsanto

800 North Lindbergh Blvd.

St. Louis, MO

United States of America, 63167

Phone:	+ 1 314 694-1000
Fax:	+1 314 694-3080
Url:	Monsanto

Record #7088

Bayer CropScience

Url:	Bayer CropScience Homepage

Description

The stacked maize line ACS-ZMØØ3-2 x MON-ØØ81Ø-6 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of PAT and Cry1Ab genes. The expression of these genes are expected to confer resistance to Lepidoptera, and tolerant to glufosinate herbicide.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #246

Zea mays - Maize, Corn, MAIZE

Record #14767

ACS-ZMØØ3-2 - Liberty Link™ maize

Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate

Show detection method(s)

Record #14750

MON-ØØ81Ø-6 - YieldGard™ maize

Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)

Show detection method(s)

Characteristics of the transformation process

Vector

pDH51, PV-ZMBK07 and PV-ZMGT10

Techniques used for the modification

Cross breeding

Genetic elements construct

Beta-lactamase gene

#14975

0.86 Kb

pUC origin of replication

#101411

2.63 Kb

CaMV 35S promoter

#100287

0.52 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.53 Kb

CaMV 35S terminator

#100290

0.20 Kb

CaMV Enhanced 35S promoter

#100366

0.61 Kb

Hsp70 intron

#100359

0.80 Kb

Cry1Ab

#14985

3.46 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

DNA insert from ACS-ZMØØ3-2 vector pDH51
Glufosinate tolerance in T25 maize due to the enzyme phosphinothricin-N-acetyltransferase (PAT). The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound. Molecular analyses shows that it has a truncated copy of the bla gene (25% of the 5' end of the bla gene is missing in T25) and an intact Ori-pUC.

DNA insert from MON-ØØ81Ø-6 vector PV-ZMBK07 and PV-ZMGT10
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. This confers resistance to Lepidoptera pests. Two constructs PV-ZMBK07 and PV-ZMGT10 were used for transformation, but molecular analyses showed no elements from the PV-ZMGT10 construct were integrated and only the elements from construct PV-ZMBK07 have been integrated into its genome. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

For additional information on this LMO, please refer to the records of the parental LMOs.

LMO characteristics

Modified traits

Resistance to antibiotics
- Ampicillin
Resistance to diseases and pests
- Insects
  - Lepidoptera (butterflies and moths)
    - European corn borer (Ostrinia nubilalis)
Resistance to herbicides
- Glufosinate

Common use(s)

Food
Feed

Detection method(s)

External link(s)

ACS-ZMØØ3-2 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

MON-ØØ81Ø-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

Additional Information

Other relevant website address or attached documents

ACS-ZMØØ3-2 x MON-ØØ81Ø-6 - OECD

ACS-ZMØØ3-2 x MON-ØØ81Ø-6 - CERA