MON-88Ø17-3 x MON-ØØ81Ø-6 - YieldGard™ VT Triple | BCH-LMO-SCBD-15374 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 12 Jul 2006 last updated: 24 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
YieldGard™ VT Triple
EN
MON88017 x MON810
MON-88Ø17-3 x MON-ØØ81Ø-6
  • - Organization: Monsanto () | BCH-CON-SCBD-14925-3
    Organization
    Monsanto ()
    800 North Lindbergh Blvd.
    St. Louis, MO
    63167, United States of America
    Phone: + 1 314 694-1000,
    Fax: +1 314 694-3080,
    Email:
A stacked insect-resistant maize derived from conventional cross-breeding of MON-88Ø17-3 and MON-ØØ81Ø-6.  Resistance to insect attack is conferred through expression of a truncated form of the cry1Ab gene and cry3Bb1 gene from Bacillus thuringiensis subsp. kurstaki HD-1 and tolerance to glyphosate-herbicides is produced through introduction of the epsps gene from Agrobacterium tumefaciens which confers tolerance to the herbicide Roundup, with the active ingredient glyphosate.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
    Crops
  • BCH-LMO-SCBD-15106-10 Living Modified Organism MON-88Ø17-3 - YieldGard™ VT™ Rootworm/RR2™ Maize
    Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glyphosate
  • BCH-LMO-SCBD-14750-19 Living Modified Organism MON-ØØ81Ø-6 - YieldGard™ maize
    Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
EN
Characteristics of the modification process
PV-ZMIR39, PV-ZMBK07 and PV-ZMGT10
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome. MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

DNA insert from MON88017 vector PV-ZMIR39
MON88017 expresses the cry3Bb1 gene encoding a Coleopteran-specific insecticidal protein to control infestation with corn root worm, and the cp4 epsps gene.

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Food
  • Feed
Additional Information
MON88017 x MON810 is resistant to insect attack and tolerant to use of the herbicide glyphosate. The cry3Bb1 gene encodes a Coleopteran-specific insecticidal protein from Bacillus thuringiensis (subsp. kumamotoensis) in order to control infestation with corn root worm, the cry1Ab gene produces the insect control protein Cry1Ab, a delta-endotoxin which is insecticidal to lepidopteran insects, and the cp4 epsps gene from the soil bacterium Agrobacterium ssp. strain CP4 provides tolerance to the herbicide glyphosate.

The cry3Bb1 gene encodes the insect control protein Cry3Bb1, a delta-endotoxin, The toxin protects the plant from the Coleopteran insects western corn rootworm (Diabrotica virgifera), northern corn rootworm (D. barberi), and Mexican corn rootworm (D. virgifera zeae).  The cry1Ab gene produces the insect control protein Cry1Ab and protects the plant against lepidopteran insects such as the european corn borer (ECB).

Cry proteins act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry3Bb1 is lethal only when eaten by Coleopteran species, including corn root worm, and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. Cry1Ab is insecticidal only when eaten by the larvae of lepidopteran insects (moths and butterflies). There are no binding sites for the delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

The cp4 epsps gene encodes for a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is highly tolerant to inhibition by glyphosate.

This line is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
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Record type Field Record(s)