CUH-CP551-8 - Papaya resistant to papaya ringspot virus | BCH-LMO-SCBD-40294 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 15 Aug 2007 last updated: 17 Jan 2014
Living Modified Organism identity
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Papaya resistant to papaya ringspot virus
EN
55-1
CUH-CP551-8
  • - Organization: Cornell University and University of Hawaii () | BCH-CON-SCBD-40290-1
    Organization
    Cornell University and University of Hawaii ()
    ,
    , United States of America
    Phone:
    Fax:
    Email:
    Website:
Papaya lines 55-1 was developed using recombinant DNA techniques to resist infection by papaya ringspot virus (PRSV), a major limiting factor in papaya production.

This papaya line was developed by inserting virus-derived sequences that encode the PRSV coat protein (CP). The introduced viral sequences do not result in the formation of any infectious particles, nor does their expression result in any disease pathology.

PRSV belongs to the potyvirus group and is an aphid-transmissible RNA virus that commonly infects papaya, causing serious disease and economic loss.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: Sunset
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  • CUH-CP631-7 - Papaya resistant to papaya ringspot virus
    | Cornell University | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Viruses, Papaya ringspot virus (PRV)), Selectable marker genes and reporter genes
Characteristics of the modification process
pGA482GG/cpPRV-4
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  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The Agrobacterium tumefaciens binary plasmid pGA482GG/cpPRSV-4 used for the transformation contained three plant-expressible genes, the PRSV CP, neo, and uidA genes. The plasmid also had two genes encoding resistance to tetracycline and gentamycin antibiotics, respectively, but their associated DNA regulatory sequences enabled expression only in bacteria. The plasmid included the right- and left-border regions derived from the A. tumefaciens T-DNA.

Expression of the PRSV CP gene was controlled by including promoter and transcription termination and polyadenylation signal sequences derived from the 35S transcript of cauliflower mosaic virus (CaMV).

In addition, the CP gene sequences were fused to the 5' untranslated sequence and the first 39 nucleotides from the cucumber mosaic virus (CMV) CP to enhance translation of the transgene mRNA. The inclusion of these additional sequences was necessary because PRSV naturally encodes its CP as part of a polyprotein and, therefore, the CP coding region normally lacks a translation initiation ATG codon.

Expression of the neo gene was under control of the promoter and terminator sequences from the nopaline synthase (nos) gene of A. tumefaciens. The second marker gene, uidA, was modified for plant expression by the addition of 35S promoter region from CaMV and the nos 3'-termination region.
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LMO characteristics
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  • Food
Detection method(s)
Southern blot analyses of genomic DNA from line 55-1 verified that it contained the PRSV CP gene, intact copies of two plant-expressible marker genes encoding NPTII and GUS, respectively, and a partial copy of the tetracycline resistance marker gene. Genomic DNA did not hybridize with probes to the gentamycin marker genes or to the origin of bacterial replication (Ori V/Tet) region. The partial tetracycline resistance gene was not expressed in plants, due both to the fact that the gene was incomplete and under the regulatory control of a bacterial promoter.
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Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 1