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Modified Organism
SYN-BTØ11-1 x SYN-IR6Ø4-5 x MON-ØØØ21-9 - Agrisure™ 3000GT Maize
Record information and status
Record ID
43635
Status
Published
Date of creation
2008-01-14 18:23 UTC (manoela.miranda@cbd.int)
Date of last update
2014-03-19 20:29 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Agrisure™ 3000GT Maize
Transformation event
BT11 x MIR604 x GA21
Unique identifier
SYN-BTØ11-1 x SYN-IR6Ø4-5 x MON-ØØØ21-9
Developer(s)
Syngenta Crop Protection AG
Basel
Switzerland
Description
The stacked maize line SYN-BTØ11-1 x SYN-IR6Ø4-5 x MON-ØØØ21-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of mCry3A, pmi, Cry1Ab, EPSPS and PAT genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate and glyphosate herbicide and the ability to use mannose as a carbon source.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize
Mannose tolerance
Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera)
Selectable marker genes and reporter genes
SYN-BTØ11-1 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Resistance to herbicides - Glufosinate
MON-ØØØ21-9 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Related LMOs
SYN-BTØ11-1 x SYN-IR6Ø4-5 - Agrisure™ CB/LL/RW maize
Mannose tolerance
Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera), Lepidoptera (butterflies and moths)
Resistance to herbicides - Glufosinate
Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pDPG434, pZM26 and pZO1502
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Rice actin 1 gene promoter
1.37 Kb
 
 
Rice actin 1, intron
0.00 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Nopaline Synthase Gene Terminator
0.24 Kb
 
 
Metallothionein-like gene promoter
2.56 Kb
 
 
mCry3A
1.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.01 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.51 Kb
 
 
Alcohol Dehydrogenase 1, intron 6
0.47 Kb
 
 
Cry1Ab
1.85 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.42 Kb
 
 
Alcohol Dehydrogenase 1, intron 2
0.18 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome.

DNA insert from Bt11 vector pZO1502
Insect-resistant and herbicide tolerant maize produced by inserting the cry1Ab gene to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from MIR604 vector pZM26
MIR604 is a genetically modified maize developed to confer field protection against corn root worms. The mcry3A gene from Bacillus thuringiensis codes for a  Bt-toxin (Cry3A), which confers resistance to western corn rootworm (Diabrotica virgifera virgifera), northern corn rootworm (Diabrotica longicornis barberi) and other related coleopteran species.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Mannose tolerance
Common use(s)
  • Food
  • Feed
Additional Information

Records referencing this document (23)
IDDescription
23record(s) found
Country's Decision or any other Communication9 records
Modified Organism1 record
Organization3 records
Risk Assessment10 records

   
   
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