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Insect-resistant and herbicide tolerant maize produced by inserting
the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki to
confer resistance to the European corn borer (Ostrinia nubilalis),
and the phosphinothricin N-acetyltransferase (PAT) encoding gene
from Streptomyces viridochromogenes to confer tolerance to
phosphinothricin (PPT) herbicide, specifically glufosinate
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
SYN-BTØ11-1 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Resistance to herbicides - Glufosinate Show detection method(s)
Alcohol Dehydrogenase 1, intron 6
Nopaline Synthase Gene Terminator
Alcohol Dehydrogenase 1, intron 2
Phosphinothricin N-acetyltransferase gene
Nopaline Synthase Gene Terminator
Information on the inserted DNA sequences:
- 35S promoters derived from cauliflower mosaic virus (CaMV) and
35S-1 originated from the CM1841 isolate of CaMV as a 500 Ddel to
Ddel fragment, subsequently converted to Sacl sites and 35S-2
originated from the Cabb-S strain of CaMV as a n Alul to Ddel
fragments (ca 425bp), whose ends were subsequently modified.
- introns derived from the maize alcohol dehydrogenase 1S gene and
were used to enhance heterologous gene expression.
- Btk gene which is an altered version of the full length cry1A(b)
gene of Bacillus thuringiensis var kurstaki HD-1. The Btk was
obtained as a 1.8kb Nco-Bg/ll fragment. The truncated Btk protein
is identical to the N-terminal 615 amino acids of the native Btk
protein of 1155 amino acids.
- pat gene (phosphinotricin acetyl transferase) cloned from the
soil microorganism, Streptomyces viridochromogenes strain
Tu494. Alteration did not result in any amino acid sequence
- nos terminator consisting of 423-678 of the nopaline synthase
gene of Agrobacterium tumefaciens plus added restriction
Plasmid pZO1502 is the vector used for the transformation of Bt
maize via a polyethylene glycol-mediated protoplast
transformation/regeneration system. This is a derivative of
plasmid pUC18. The plasmid has a molecular weight of 2.7 kb and
contains the following sequences:
- the prokaryotic gene bla (also called ampR) under a procaryotic
promoter encoding β-lactamase, which confers resistance to
ampicillin; it is used as a bacterial selectable marker;
- the gene lac Z, encoding a portion of a β-galactosidase, this
gene is not functional;
- the pUC origin of replication derived from the plasmid pBR 322
carrying a mutation.
- Resistance to antibiotics
- Resistance to diseases and pests
- Lepidoptera (butterflies and moths)
- European corn borer (Ostrinia nubilalis)
- Resistance to herbicides
The coding sequences of the Bt10 event are identical to those
previously reported for the registered event, Bt11. Syngenta
provided data that all of the nucleotides in the coding region are
identical in Bt10 and Bt11. The expressed proteins are Cry1Ab and
the inert marker PAT for herbicide tolerance.