Bt-10 Maize | BCH-LMO-SCBD-45400 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 11 Jun 2008 last updated: 02 Nov 2012
Living Modified Organism identity
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Bt-10 Maize
Insect-resistant and herbicide tolerant maize produced by inserting the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces viridochromogenes to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • SYN-BTØ11-1 - YieldGard™ maize
    | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate)
Characteristics of the modification process
  • Direct DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-14975-5 Beta-lactamase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Ampicillin)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-103625-3 Alcohol dehydrogenase 1, intron 6 | Zea mays (Maize, Corn, MAIZE)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-103867-2 Alcohol dehydrogenase 1, intron 2 | Zea mays (Maize, Corn, MAIZE)
Information on the inserted DNA sequences:
- 35S promoters derived from cauliflower mosaic virus (CaMV) and 35S-1 originated from the CM1841 isolate of CaMV as a 500 Ddel to Ddel fragment, subsequently converted to Sacl sites and 35S-2 originated from the Cabb-S strain of CaMV as a n Alul to Ddel fragments (ca 425bp), whose ends were subsequently modified.
- introns derived from the maize alcohol dehydrogenase 1S gene and were used to enhance heterologous gene expression.
- Btk gene which is an altered version of the full length cry1A(b) gene of Bacillus thuringiensis var kurstaki HD-1. The Btk was obtained as a 1.8kb Nco-Bg/ll fragment. The truncated Btk protein is identical to the N-terminal 615 amino acids of the native Btk protein of 1155 amino acids.
- pat gene (phosphinotricin acetyl transferase) cloned from the soil microorganism, Streptomyces viridochromogenes strain Tu494.  Alteration did not result in any amino acid sequence changes.
- nos terminator consisting of 423-678 of the nopaline synthase gene of Agrobacterium tumefaciens plus added restriction sites.

Vector information:
Plasmid pZO1502 is the vector used for the transformation of Bt maize via a polyethylene glycol-mediated protoplast transformation/regeneration system.  This is a derivative of plasmid pUC18. The plasmid has a molecular weight of 2.7 kb and contains the following sequences:
- the prokaryotic gene bla (also called ampR) under a procaryotic promoter encoding β-lactamase, which confers resistance to ampicillin; it is used as a bacterial selectable marker; 
- the gene lac Z, encoding a portion of a β-galactosidase, this gene is not functional;
- the pUC origin of replication derived from the plasmid pBR 322 carrying a mutation.
LMO characteristics
Detection method(s)
Additional Information
The coding sequences of the Bt10 event are identical to those previously reported for the registered event, Bt11. Syngenta provided data that all of the nucleotides in the coding region are identical in Bt10 and Bt11. The expressed proteins are Cry1Ab and the inert marker PAT for herbicide tolerance.
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Country's Decision or any other Communication Living modified organism(s) 2
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Risk Assessment generated by a regulatory process Living modified organism(s) 1