Wheat modified for resistance against corn smut (KP4-Greina 16) | BCH-LMO-SCBD-48078 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
published: 09 Jan 2009 last updated: 13 Sep 2013
Living Modified Organism identity
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Wheat modified for resistance against corn smut (KP4-Greina 16)
EN
KP4-Greina 16
No
The kp4 gene is derived from the genome of a double-stranded RNA virus (Ustilago Maydis Virus 4, UmV4), which is present in the tissue of certain fungal strains of corn smut (Ustilago maydis). The expression of the kp4 gene (KP4, killer protein 4) in Ustilago maydis cells leads to a reversible inhibition of hyphal growth, but does not kill off competing strains. It is expected that plants expressing the KP4 protein are less susceptible to damage caused by the phytopathogenic fungi Ustilago species. Expression of the kp4 gene is under control of the ubiquitin promoter of the uidA gene from Zea mays and the t35S terminator of CaMV.

The bar gene derives from Streptomyces hygroscopicus and confers herbicide resistance against phosphinothricine (Bialaphos). The gene was used as a transformation marker. Expression of the bar gene is controlled by the actin promoter of Oryza sativa and the t35S terminator of CaMV.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Wheat Cultivar/breeding line Greina
EN
Characteristics of the modification process
pUbi::kp4 (pUC19 derivate) and pAct::bar (pUC19 derivate)
EN
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
EN
LMO characteristics
EN
  • Food
Detection method(s)
EN
Additional Information
LMO authorized for a specific field trial in Germany.
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1