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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Wheat modified for resistance against corn smut (KP4-Greina 16)
EN
KP4-Greina 16
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Person:Dr. Christof Sautter, Institute of Plant SciencesUniversitätstrasse 2 8092 Zürich SWITZERLAND,
,Phone: +41-44-632-5713,Fax: +41-44-632-1044,Website: http://www.ethz.ch/index_EN,Related OrganizationSwiss Federal Institute of Technology Zürich (ETH) ()Universitätstrasse 2 8092 Zürich SWITZERLAND,
,Phone: +41-44-632-5713,Fax: +41-44-632-1044,Website: http://www.ethz.ch/index_EN,
The kp4 gene is derived from the genome of a double-stranded RNA virus (Ustilago Maydis Virus 4, UmV4), which is present in the tissue of certain fungal strains of corn smut (Ustilago maydis). The expression of the kp4 gene (KP4, killer protein 4) in Ustilago maydis cells leads to a reversible inhibition of hyphal growth, but does not kill off competing strains. It is expected that plants expressing the KP4 protein are less susceptible to damage caused by the phytopathogenic fungi Ustilago species. Expression of the kp4 gene is under control of the ubiquitin promoter of the uidA gene from Zea mays and the t35S terminator of CaMV.
The bar gene derives from Streptomyces hygroscopicus and confers herbicide resistance against phosphinothricine (Bialaphos). The gene was used as a transformation marker. Expression of the bar gene is controlled by the actin promoter of Oryza sativa and the t35S terminator of CaMV.
EN
The bar gene derives from Streptomyces hygroscopicus and confers herbicide resistance against phosphinothricine (Bialaphos). The gene was used as a transformation marker. Expression of the bar gene is controlled by the actin promoter of Oryza sativa and the t35S terminator of CaMV.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-45396-4 Organism Triticum aestivum (Wheat)Crops
Wheat Cultivar/breeding line Greina
EN
pUbi::kp4 (pUC19 derivate) and pAct::bar (pUC19 derivate)
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- Biolistic / Particle gun
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-47790-4 Killer Protein 4 | Ustilago maydis virus H1Protein coding sequence | Resistance to diseases and pests (Fungi)
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BCH-GENE-SCBD-14975-5 Beta-lactamase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Ampicillin)
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
EN
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- Food
EN
LMO authorized for a specific field trial in Germany.
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- JRC Biotechnology and GMOs [ English ]
- Verhalten von transgenen KP4-Weizen Varietäten im Feld ("Behaviour of KP4 transgenic wheat varieties in the field") - in German [ English ]
- Development of the particle inflow gun for DNA delivery into plant cells..pdf [ English ]
- Antifungal activity of a virally encoded gene in transgenic wheat [ English ]
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