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Potato modified for resistance to Phytophthora infestans
BASF Plant Science GmbH
The Rpi-blb1 and Rpi-blb2 genes inserted in these genetically
modified potatoes should confer increased resistance to
Phytophthora infestans. Rpi-blb1 and Rpi-blb2 code for proteins of
the NBS-LRR (nucleotide binding site-leucine rich repeat)
The ahas gene inserted in the potato plants imparts tolerance to
the herbicidal active substance Imazamox to the shoots during the
selection process in cell culture.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Vector VCPMA19 derived from pPZP200
- Agrobacterium-mediated DNA transfer
Phytophthora infestans Resistance gene 2 Promoter
Phytophthora infestans Resistance gene 2
Phytophthora infestans Resistance gene 2 Terminator
Phytophthora infestans Resistance gene 1 Promoter
Phytophthora infestans Resistance gene 1
Phytophthora infestans Resistance gene 1 Terminator
Nopaline Synthase Gene Promoter
Acetohydroxy acid synthase gene
Nopaline Synthase Gene Terminator
- Resistance to diseases and pests
- Phytophthora infestans resistance
- Resistance to herbicides
Observed gene expression in the transgenic plants
In the genetically modified lines a low level of expression of both
Rpi-blb1 and Rpi-blb2 has been demonstrated by real-time PCR
analysis in leaves, stems, tubers and roots. In flowers a low
expression of Rpi-blb2 can be detected, while Rpi-blb1 is expressed
at even lower levels or not at all.
The ahas gene is controlled by the nos promoter, which is known to
result in low expression levels in all parts of the plant.
Expression of the ahas gene in the genetically modified potato
material has been demonstrated in tissue culture by survival on
media containing Imazamox.
Derivate of pPZP200. Reference: Hajdukiewicz et al (1994) Plant
Mol. Biol., 25, 989-994