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BASF Plant Science GmbH
The potato was modified for resistance to Phytophthora
infestans through the expression of the resistance (R) genes
Rpi-blb1 and Rpi-blb2 from a wild relative
Solanum bulbocastaneum. The R genes encode
nucleotide-binding site-leucine rich repeat type proteins and play
a role in host defence against the pathogenic fungus.
Additionally, a selectable marker, Arabidopsis thaliana
acetohydroxyacid synthase, was included for imidazolinone selection
during transformation. The enzyme carries a point mutation, S653N,
which confers herbicide tolerance.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Vector VCPMA16 derived from pPZP200
- Agrobacterium-mediated DNA transfer
Phytophthora infestans Resistance gene 2 Promoter
Phytophthora infestans Resistance gene 2
Phytophthora infestans Resistance gene 2 Terminator
Phytophthora infestans Resistance gene 1 Promoter
Phytophthora infestans Resistance gene 1
Phytophthora infestans Resistance gene 1 Terminator
Nopaline Synthase Gene Promoter
Acetohydroxy acid synthase gene
Nopaline Synthase Gene Terminator
The insertion contains three gene expression cassettes: Solanum
bulbocastaneum Phytophthora infestans resistance gene 1
(Rpi-blb1), Solanum bulbocastaneum Phytophthora
infestans resistance gene 2 (Rpi-blb2) and
Arabidopsis thaliana acetohydroxyacid synthase
Transcription of Rpi-blb1 and Rpi-blb2 are under
control of the native promoters and terminators. Transcription of
ahas is under control of the Agrobacterium
tumefaciens nopaline synthase promoter and terminator. The R
gene cassettes were originally excised from the donor genome as
genomic fragments and inserted into the vector.
The coding sequence of ahas contains a point mutation
S653N (serine to asparagine at amino acid position 653), which
confers herbicide tolerance.
- Resistance to antibiotics
- Resistance to diseases and pests
- Resistance to herbicides
- Phytophthora infestans resistance
Low level of expression of both Rpi-blb1 and
Rpi-blb2 was detected by real-time PCR analysis in leaves,
stems, tubers and roots. In flowers, low expression of Rpi-blb2
was detected. However, the expression of Rpi-blb1 was
not detected in floral tissues.
Due to the nos promoter, low expression levels in all
parts of the plant are expected for ahas. Expression of
ahas was essential for the selection of transformants
during tissue culturing.
Derivate of pPZP200. Reference: Hajdukiewicz et al (1994) Plant
Mol. Biol., 25, 989-994.