eCry3.1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU) | BCH-GENE-SCBD-104789 | Genetic element | Biosafety Clearing-House

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Genetic element (GENE)

published: 22 Mar 2013 last updated: 24 Feb 2014
General information
eCry3.1Ab
EN
CS-eCry3_1Ab-BACTU
EN
Protein coding sequence
Yes
Donor organism
EN
Characteristics of the protein coding sequence
eCry3.1Ab delta-endotoxin
EN
An engineered Cry gene active against certain corn rootworm (Diabrotica) species . The gene ecry3.1Ab consists of a fusion between the 5′ end (Domain I, Domain II and 15 amino acids of Domain III) of a modified Cry3A gene (mcry3A) and the 3′ end (Domain III and Variable Region 6) of a synthetic Cry1Ab gene

Upstream of the mcry3A domain, the gene ecry3.1Ab carries a 67-bp-long oligomer extension at its 5′ end, which was introduced during the engineering of the variable regions and is translated into the following 22 amino acid residues: MTSNGRQCAGIRPYDGRQQHRG. The next 459 amino acid residues are identical to a portion of mCry3A, followed by 172 residues that are identical to a portion of Cry1Ab.
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Introduced or modified genetic element(s) 37
Living Modified Organism Genetic elements construct 37