2-deoxyglucose-6-phosphate phosphatase 1 | Saccharomyces cerevisiae (Yeast, YEASX) | BCH-GENE-SCBD-111081 | Genetic element | Biosafety Clearing-House

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Genetic element (GENE)

published: 25 Oct 2016 last updated: 02 Aug 2022
General information
2-deoxyglucose-6-phosphate phosphatase 1
EN
  • DOG1
    EN
CS-dog1-YEASX
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Protein coding sequence
No
Donor organism
EN
Characteristics of the protein coding sequence
2-desoxyglucose-6-phosphate phosphatase
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In plant cells a glucose analog, 2-deoxyglucose (2-DOG), is phosphorylated by hexokinase to form 2-DOG-6-phosphate, which competes with glucose-6-phosphate causing cell death through the inhibition of glycolysis, protein synthesis, cell wall polysaccharide synthesis and also protein glycosylation.

DOGR1 gene, which has been isolated from Saccharomyces cerevisiae strain S288C, encodes 2-deoxyglucose-6-phosphate. The enzyme has the ability to convert toxic 2-DOG-6-phosphate to non-toxic products through dephosphorylation.

Therefore, transformed plant cells which carry the DOGR1 gene can be selected on medium containing 2-DOG.
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