2-deoxyglucose-6-phosphate phosphatase gene | BCH-GENE-SCBD-111081 | Genetic element | Biosafety Clearing-House

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Genetic element (GENE)

last updated: 25 Oct 2016
General information
2-deoxyglucose-6-phosphate phosphatase gene
EN
CS-DOGR1-YEASX
EN
Protein coding sequence
No
Donor organism
EN
Characteristics of the protein coding sequence
2-desoxyglucose-6-phosphate phosphatase
EN
In plant cells a glucose analog, 2-deoxyglucose (2-DOG), is phosphorylated by hexokinase to form 2-DOG-6-phosphate, which competes with glucose-6-phosphate causing cell death through the inhibition of glycolysis, protein synthesis, cell wall polysaccharide synthesis and also protein glycosylation.

DOGR1 gene, which has been isolated from Saccharomyces cerevisiae strain S288C, encodes 2-deoxyglucose-6-phosphate. The enzyme has the ability to convert toxic 2-DOG-6-phosphate to non-toxic products through dephosphorylation.

Therefore, transformed plant cells which carry the DOGR1 gene can be selected on medium containing 2-DOG.
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Introduced or modified genetic element(s) 1
Living Modified Organism Genetic elements construct 1