CDC-FLØØ1-2 - CDC Triffid flax modified for herbicide resistance | BCH-LMO-SCBD-14768 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 15 Jan 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
CDC Triffid flax modified for herbicide resistance
Linseed tolerant to the herbicide sufonylurea through insertion of the acetolactate synthase (als) gene.  Neomycin phosphotransferase II (neo) confers resistance to the antibiotic kanamycin and the nos gene codes for nopaline synthase; these were used as selectable markers.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
pGH6 derived from pGV3850
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-15171-5 Nopaline Synthase Gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-103932-4 Acetohydroxy acid synthase gene promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-103933-4 Acetohydroxy acid synthase gene terminator | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
LMO characteristics
  • Food
  • Feed
Additional Information
Sulfonylurea herbicides, such as triasulfuron and metsulfuron-methyl, target and bind to the enzyme acetolactate synthase (ALS) thereby inhibiting the biosynthesis of the branched chain amino acids valine, leucine and isoleucine and resulting in the accumulation of toxic levels of alpha-ketoglutarate.

In addition to its native ALS gene, CDC Triffid contains an als gene from a chlorsulfuron tolerant line of Arabidopsis thaliana. This variant als gene differs from the wild type A. thaliana gene by one nucleotide and the resulting ALS enzyme differs by one amino acid from the wild type ALS enzyme. The inserted als gene is linked to its native promoter and terminator.

Enzyme extracts from CDC Triffid exhibited a slightly higher ALS activity compared to its non-modified counterpart cv. Norlin. Whereas the statistical significance of this higher activity could not be verified, it may be expected due to the presence of at least two additional copies of the als gene in CDC Triffid.