BCS-GHØØ2-5 × ACS-GHØØ1-3 - GlyTol™ Liberty Link™ cotton | BCH-LMO-SCBD-101250 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
published: 23 Jun 2010 last updated: 24 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
GlyTol™ Liberty Link™ cotton
GHB614 x LLCotton25 (a.k.a. GT x LL or Glytol x LL)
GHB614 × LLCotton25 has been obtained by cross breeding between two living modified cotton parental lines: GHB614 and LLCotton25.

The stacked LMO GHB614 × LLCotton25 inherited the following traits from the parental lines: tolerance to glyphosate from GHB614 and tolerance to glufosinate ammonium herbicides from LLCotton25.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
pTEM2 and pGSV71
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-46333-8 5-enolpyruvylshikimate-3-phosphate synthase | Zea mays (Maize, Corn, MAIZE)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-104647-3 Histone H4 gene Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-104648-2 Histone H3 Gene II intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-101419-4 Optimized Transit Peptide
    Transit signal
  • BCH-GENE-SCBD-104646-4 Histone H4 gene 3' UTR | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
DNA insert from GHB614 vector pTEM2
The T-DNA cassette from this LM cotton line contained the 2mepsps coding sequence which confers tolerance to the glyphosate herbicide.

DNA insert from LLCotton25 vector pGSV71
The T-DNA cassette from teh LLCotton25 line contined the coding sequence for the bar gene which confers tolerance to the glufosinate herbicide.

For additional information on this LMO, please refer to the records of the parental LMO.
LMO characteristics
  • Food
  • Feed
  • Fiber/textile
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 45
Risk Assessment generated by a regulatory process Living modified organism(s) 45
Living Modified Organism Related LMO(s) 1