MON-ØØØ21-9 × MON-ØØ81Ø-6 - Roundup Ready™ YieldGard™ maize | BCH-LMO-SCBD-14892 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 20 Feb 2018
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ YieldGard™ maize
GA21 x MON810
MON-ØØØ21-9 × MON-ØØ81Ø-6
This LMO is a stacked insect-resistant and glyphosate-tolerant maize derived from conventional cross-breeding of MON-ØØØ21-9 and MON-ØØ81Ø-6. 
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
  • BCH-LMO-SCBD-14750-19 Living Modified Organism MON-ØØ81Ø-6 - YieldGard™ maize
    Monsanto | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis))
  • BCH-LMO-SCBD-14794-18 Living Modified Organism MON-ØØØ21-9 - Roundup Ready™ maize
    Monsanto | Resistance to herbicides (Glyphosate)
Characteristics of the modification process
pDPG434, PV-ZMBK07 and PV-ZMGT10
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from GA21, vector pDPG434:

The GA21 line of maize was genetically engineered, by particle acceleration (biolistic) transformation, to be tolerant of glyphosate-containing herbicides.

DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10:

MON810 contains a truncated portion of a synthetic form of the cry1Ab gene. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
  • Food
  • Feed
  • Biofuel
Detection method(s)
GA21 contains a single single insertion site with 3 complete copies of mEPSPS cassette plus 3 incomplete copies as follows: (1) a mEPSPS gene cassette, truncated at the 5' end of the rice actin promoter sequence; (2) three complete internal mEPSPS gene cassettes; (3) a partial mEPSPS gene cassette containing the promoter, intron, otp, and a partial mEPSPS coding sequence terminating in a stop codon; and (4) an additional partial gene cassette at the 3' end containing only the rice actin promoter and 5' mRNA leader sequence, but truncating before the start of the rice actin intron, followed by corn genomic DNA.

MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 6
Country's Decision or any other Communication Living modified organism(s) 5
Risk Assessment generated by a regulatory process Living modified organism(s) 4