Loading...
You are viewing a DELETED record.
This record information is displayed for reference purpose only and should be not used.
This document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Genetic element (GENE)
last updated: 02 Feb 2023
Vpb4Da2
EN
-
Vip4Da2EN
CS-vpb4da2-BACTU
EN
Protein coding sequence
No
-
BCH-ORGA-SCBD-45614-11 Organism Bacillus thuringiensis (Bt, Bacillus, BACTU)Bacteria
Bacillus thuringiensis stain EG6657
EN
The VPD4Da2 protein has a similar mode of action to other Bt proteins: proteolytic activation, binding to a receptor in the midgut, oligomerization in the membrane, pore formation and cell death. This cellular damage leads to death of the insect. However, the protein forms beta-barrel pores rather than the alpha-helical transmembrane pores Bt proteins form.
EN
The Vpb4Da2 structure depicts an overall architecture of six structural domains:
- Domain 1 (amino acids 1–263), which encompasses a characteristic PA14 domain, adopts a conformation of ten anti-parallel β-strands, six small α-helices, and binds two adjacent calcium ions visible in the structure
- Domain 2 (amino acids 264–483) consists of a β-hairpin motif, seven anti-parallel β-strands, a partially structured long loop, and three additional α-helices.
- Domain 3 (amino acids 484–593) has a β-sandwich fold of seven β-strands and four α-helices.
- Domain 4 (amino acids 594–730) consists of β-strands exhibiting a classical β-jelly roll fold and harboring a calcium ion.
- Domain 5 (amino acids 731–820) has an immunoglobulin-like fold of six β-strands and one α-helix.
- Domain 6 (amino acids 821–937), like domain 5, adopts an immunoglobulin-like fold with seven β-strands and two α-helices.
- A new Bacillus thuringiensis protein for Western corn rootworm control.pdf [ English ]
- Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm.pdf [ English ]
- Food and feed safety of the Bacillus thuringiensis derived protein Vpb4Da2, a novel protein for control of western corn rootworm.pdf [ English ]
- GenBank - Synthetic construct Vip4Da2 gene, complete cds [ English ]
Loading...
