Cry1A.105 | BCH-GENE-SCBD-43771 | Genetic element | Biosafety Clearing-House


Genetic element (GENE)

last updated: 22 Jun 2021
General information
Protein coding sequence
Donor organism
Characteristics of the protein coding sequence
Cry1A.105 delta-endotoxin
Cry1A.105 is a full-length, insecticidal protein consisting of 1177 amino acids with a molecular weight (MW) of 133 kDa. It is a chimeric protein that consists of domains I and II from Cry1Ab or Cry1Ac1, domain III from Cry1F, and the C-terminal domain
from Cry1Ac.

Cry1A.105 was designed using domain exchange strategy to achieve high levels of activity against target lepidopteran insect pests. The domains I and II of Cry1A.105 are 100% identical to the respective domains of Cry1Ab or Cry1Ac. The domain III of Cry1A.105 is 99% identical to the domain III of Cry1F. The C-terminal region of Cry1A.105 is 100% identical to that of Cry1Ac.

Cry1Ac, Cry1Ab and Cry1F are all well known and well characterized insecticidal proteins derived from the soil bacterium Bacillus thuringiensis (Bt).The overall amino acid sequence identity of Cry1A.105 to Cry1Ac, Cry1Ab, and Cry1F is 93.6%, 90.0%, and 76.7%, respectively
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Introduced or modified genetic element(s) 85
Living Modified Organism Genetic elements construct 84
Laboratory for detection and identification of LMOs Genetic element(s) detectable by the laboratory 2