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Living Modified Organism
(LMO)
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Potato with altered growth and tuber quality
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Potato transformed with B33-Apy1-RNAi 1331 (3 lines: #3, #10 und #25)
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Organization:Max-Planck-Institute of Molecular Plant Physiology ()Wissenschaftspark Golm Am Mühlenberg 1 14476 Potsdam - Golm Germany,
,Phone:Fax:Email:Website:
Potato plant was modified with an inverted repeat RNAi construct of the Solanum tuberosum Apyrase gene along with an nptII expression cassette. The RNAi construct results in the degradation of native apyrase RNA transcript thus reducing the expression of the enzyme. This results in the modfied growth of potato tubers expressing the gene. The nptII gene conferred resistance to kanamycin and was used as a selection marker.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
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B33-Apy1-RNAi 1331
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-48365-2 Apyrase-gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | Other growth, development and product quality
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100273-4 B33 gene promotor | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103123-6 Pyruvate orthophosphate dikinase, Intron 3 | Flaveria trinervia (Clustered Yellowtops, speedyweed, flaveria, yellow twinstem)Intron
Using the RNAi construct, the RNA of a part of the apyrase gene is coded in sense and antisense orientation, so that the sense and antisense RNA are separated by a sufficiently long spacing sequence (RNA of the Pdk intron from Flaveria trinervia).
In the present case RNAi synthesis takes place under the control of the B33 promoter specifically in the potato tubers. The sense and antisense RNAi fractions form a double strand, the individual strands of which are linked by a hairpin loop.
This double-stranded RNA is recognised by specific enzymes in the plant cell and is fractionated into small fragments. These bind to the mRNA of the respective gene (the apyrase gene) and mediate the decomposition of the RNA by the same enzymes.
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In the present case RNAi synthesis takes place under the control of the B33 promoter specifically in the potato tubers. The sense and antisense RNAi fractions form a double strand, the individual strands of which are linked by a hairpin loop.
This double-stranded RNA is recognised by specific enzymes in the plant cell and is fractionated into small fragments. These bind to the mRNA of the respective gene (the apyrase gene) and mediate the decomposition of the RNA by the same enzymes.
Silencing of the endogenous apyrase gene applying RNAi technology.
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- Food
- Research
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- Riewe et al. (2008) A cell wall-bound adenosine nucleosidase is involved in the salvage of extracellular ATP in Solanum tuberosum. Plant Cell Physiology 49 (10): 1572-1579. [ English ]
- Riewe et al. (2008) The potato-specific apyrase is apoplastically localized and has influence on gene expression, growth and development. Plant Physiol. 147 (3): 1092-1109. [ English ]
- Windsor, B. et al. (2003) Multiherbicide tolerance conferred by AtPgp1 and apyrase overexpression in Arabidopsis thaliana. Nat. Biotechnol. 21: 428-433. [ English ]
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