BCS-GHØØ5-8 - Insect-protected, herbicide-tolerant cotton | BCH-LMO-SCBD-101898 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 28 Jan 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant and lepidoptera-resistant cotton
EN
GHB119
Yes
BCS-GHØØ5-8
The introduced lepidoptera resistance trait in GHB119 cotton is conferred by the insecticidal crystal protein, Cry2Ae, encoded by the cry2Ae gene, from the common soil bacterium, Bacillus thuringiensis subsp. dakota (B.t. dakota). The Cry2Ae protein is effective in controlling lepidopteran plant feeding larvae such as cotton bollworm larvae (CBW, Helicoverpa zea), tobacco budworm larvae (TBW, Heliothis virescens) and fall armyworm larvae (FAW, Spodoptera frugiperda), which are common pests of cotton.

The herbicide tolerance trait (OECD, 1999 M-204493-01-1) is based upon the bar gene, a bialaphos resistance gene, isolated from the soil microorganism, Streptomyces hygroscopicus. The bar gene, when expressed, enables the production of the enzyme, Phosphinothricin-Acetyl-Transferase (PAT) that acetylates L-glufosinate ammonium and thereby confers tolerance to herbicides based upon glufosinate ammonium.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pTEM12 derived from pGSC1700
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-101900-6 CsVMV promoter | Cassava vein mosaic virus (Cassava vein mosaic virus, CVMV, CsVMV)
    Promoter
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)
    Leader
  • BCH-GENE-SCBD-101902-4 rbcS Transit Peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-101895-8 Cry2Ae | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), Fall armyworm (Spodoptera frugiperda))
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
Southern Blot analysis indicated that a single copy of the insert is present in the GHB119 line and a configuration that correspond to that in the original vector. No insertion of plasmid backbone sequences were detected, however three ORF were present at the junctions of the integration site but do not have the required regulatory elements for functionality.

The vector pTEM12 is derived from pGSC1700 which was constructed in E. coli and thereafter transferred to a suitable Agrobacterium tumefaciens strain.
EN
LMO characteristics
EN
  • Food
  • Feed
  • Fiber/textile
  • Food (Industrial)
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Recipient Organism” or “Parental Organisms 9
Risk Assessment generated by a regulatory process Living modified organism(s) 10
Country's Decision or any other Communication Living modified organism(s) 11
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 3
Risk Assessment generated by an independent or non-regulatory process Living modified organism(s) 1

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