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Living Modified Organism
(LMO)
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Petunia modified for antibiotic resistance
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T16
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- Person: Universität Rostock | BCH-CON-DE-48069-2 This document has been updated. This is not the latest published version. Click here to view the latest version of the record.Person:Universität Rostock, Agrobiotechnology - Faculty of Agricultural and Environmental SciencesJustus-von-Liebig-Weg 6Rostock, Mecklenburg-Vorpommern
18059, GermanyPhone: +49 381 498 ext 3080,Fax: +49 381 498 ext 3082,Email: dekan.auf@uni-rostock.de,
Petunia hybrida T16 are transplastomic plants that express the aadA and the uidA gene. The LMO was constructed for an evaluation of pollen mediated transfer rates of transgenes located in the plastid genome in field grown transgenic petunia.
While the aadA gene is used as a selection marker (resistance towards streptomycine and spectinomycine is conferred) the expression of the uidA gene in plastids enables visualisation of transgenic plastids. Furthermore the homologous recombination of the insert into the plastidial genome was mediated by the rbcL- and the accD-gene so that exclusively plastids were transformed but not the nuclear genome. Since pollen does not possess plastids a vertical gene transfers seems impractical.
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While the aadA gene is used as a selection marker (resistance towards streptomycine and spectinomycine is conferred) the expression of the uidA gene in plastids enables visualisation of transgenic plastids. Furthermore the homologous recombination of the insert into the plastidial genome was mediated by the rbcL- and the accD-gene so that exclusively plastids were transformed but not the nuclear genome. Since pollen does not possess plastids a vertical gene transfers seems impractical.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12107-5 Organism Petunia hybrida (Petunia, PETHY)Crops
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pUM73(AD)
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- Biolistic / Particle gun
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-102611-2 rbcL ribosomal binding site | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence
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BCH-GENE-SCBD-102612-3 16S rRNA gene promoter | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Promoter
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BCH-GENE-SCBD-102616-2 Photosystem II 44 kDa protein (psbC) terminator | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Terminator
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BCH-GENE-SCBD-102615-4 16S rRNA gene promoter | Nicotiana tabacum (Tobacco, TOBAC )Promoter
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BCH-GENE-SCBD-102614-3 D1 thylakoid membrane protein (psbA) gene terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
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BCH-GENE-SCBD-102613-4 Acetyl-CoA carboxylase large subunit | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence | Homologous recombination
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BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Streptomycin)
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
The accD and the rbcL genes encompass the insert and enable homologous recombination into plastidial DNA of Petunia hybrida Juss. T16.
The uidA gene is used for the colorimetric and fluorimetric detection of transformed plastids.
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The uidA gene is used for the colorimetric and fluorimetric detection of transformed plastids.
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- Research
PCR-based methods need to be developed on information about the transformation vector.
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