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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Wheat modified for increased protein content
EN
XAP Wheat
No
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Organization:Institut für Pflanzengenetik und Kulturpflanzenforschung ()Corrensstrasse 3Gatersleben,
06466 , GermanyPhone: +49 (0)39482 5-0 ,Fax: +49 (0) 39482 5139,Email: info@ipk-gatersleben.de,Website: http://www.ipk-gatersleben.de,
The endosperm-specific expression of the amino acid permease VfAAp1 in XAP wheat leads to increased amino acid uptake into the endosperm that might be used in the amino acid biosynthesis process. Therefore, an increase in the protein content in the wheat grains is expected. Additionally, wheat transformed with VfAAp1 is likely to exhibit early flowering.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-45396-4 Organism Triticum aestivum (Wheat)Crops
Cultivar/ Breeding line: Certo
EN
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High-protein, herbicide resistant Barley HOSUT| Changes in physiology and/or production (Yield), Changes in quality and/or metabolite content (Carbohydrates), Resistance to herbicides (Glufosinate)
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Wheat modified for increased protein content| Changes in quality and/or metabolite content (Protein and amino acids), Resistance to antibiotics (Ampicillin), Resistance to herbicides (Glufosinate)
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High-protein, antibiotic and herbicide resistant wheat SUTAP60| Changes in quality and/or metabolite content (Carbohydrates, Protein and amino acids), Resistance to antibiotics (Ampicillin), Resistance to herbicides (Glufosinate)
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High-protein, antibiotic and herbicide resistant wheat SUTAP69| Changes in quality and/or metabolite content (Carbohydrates, Protein and amino acids), Resistance to antibiotics (Ampicillin), Resistance to herbicides (Glufosinate)
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High-protein, antibiotic and herbicide resistant wheat SUTAP78| Changes in quality and/or metabolite content (Carbohydrates, Protein and amino acids), Resistance to antibiotics (Ampicillin), Resistance to herbicides (Glufosinate)
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High-protein, herbicide resistant wheat HOSUT| Changes in physiology and/or production (Yield), Changes in quality and/or metabolite content (Carbohydrates, Protein and amino acids), Resistance to herbicides (Glufosinate)
pUC19/XAP and co-transformation of pJFBar
EN
- Biolistic / Particle gun
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2.376 kb
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1.427 kb
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0.727 kb
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0.000 kb
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0.000 kb
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0.000 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-102884-2 High molecular weight glutenin subunit 1Ax1 gene promoter | Triticum aestivum (Wheat)Promoter
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-14975-5 Beta-lactamase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Ampicillin)
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BCH-GENE-SCBD-48368-4 Amino acid permease 1 gene | Vicia faba (Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean)Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
By transformation of wheat with the vector pUC19/XAP the amino acid permease VfAAP1 was introduced into the wheat genome. Since VfAAP1 is put under the control of the endopserm-specific 1Ax 1 promoter and the octopine synthase terminator from Agrobacterium tumefaciens VfAAP1 is exclusively expressed within the endosperm during the phase of increased protein biosynthesis in wheat grains. This is meant to optimize the amino acid supply for increased levels of protein biosynthesis.
Also the integration of the beta lactamase gene from pUC19 was observed. This gene mediates resistance to bacteria towards ampicillin and was used as a selection marker prior to transformation of wheat plants. Simultaneously, the vector pJFBar was co-transformed to mediate resistance towards phospinotricine (PPT, Glufosinat) to be used as a selective marker system.
EN
Also the integration of the beta lactamase gene from pUC19 was observed. This gene mediates resistance to bacteria towards ampicillin and was used as a selection marker prior to transformation of wheat plants. Simultaneously, the vector pJFBar was co-transformed to mediate resistance towards phospinotricine (PPT, Glufosinat) to be used as a selective marker system.
EN
- Research
EN
EN
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