GTL-GFM311-6 - Cotton modified for insect resistance | BCH-LMO-SCBD-103111 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 22 Feb 2012 last updated: 26 Jan 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Cotton modified for insect resistance
This modified cotton contains the insecticidal cry1Ab/Ac gene which imparts resistance against Lepidoptera.

NOTE: This LMO was formerly referred to with the UID GTL-GFM311-7.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
pGBI4AB derived from pBI121
  • Other (Pollen Tube Pathway method (Chinese Patent))
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-103109-5 Cry1Ab/Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.))
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)
    Protein coding sequence | Selectable marker genes and reporter genes
This modified cotton contains the insecticidal cry1Ab/Ac gene which imparts resistance against Lepidoptera. The LMO also contains the selectable markers nptII to isolate transformed seedlings and a GUS gene cassette as a reporter gene.

Bt-cotton was generated by Pollen-Tube-Pathway mediated plasmid-DNA delivary into the embryo-sacs/egg apparatus. Following integration with the fertilized egg, the transformed embryos were detected by the nptII kanamycin selection system of the germinating seedlings. Putative transformants were analysed through Southerns and assayed for the level of insect resistance

The Bt transgene in the converted Indian inbred lines behaves as a single dominant Mendelian factor and is stably integrated in the plant genome.

The cry1Ab/Ac gene derived from Bacillus thuringiensis subsp. kurstaki (B.t.k.).
LMO characteristics
  • Feed
Detection method(s)
Immunological and DNA based detection methods have been developed for detection of cry1Ab-Ac protein. Central Institute of Cotton Research, Nagpur is the referral laboratory for the same.
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1