SYN-Ø53Ø7-1 - Agrisure® Duracade™ Maize | BCH-LMO-SCBD-104791 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 17 Apr 2020
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Agrisure® Duracade™ Maize
Event 5307
Event 5307 corn plants contain the transgene ecry3.1Ab encoding a novel rootworm-control protein, eCry3.1Ab, and the transgene pmi encoding the enzyme phosphomannose isomerase (PMI).

The eCry3.1Ab protein is an engineered chimera of the modified Cry3A (mCry3A) and Cry1Ab proteins, members of a class of insecticidal proteins derived from Bacillus thuringiensis(Bt). The gene pmi was obtained from Escherichia colistrain K-12 and the protein it encodes was utilized as a plant selectable marker during development of 5307 corn.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Corn line: NP2222
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-104788-2 Cestrum Yellow Leaf Curling Virus promoter | Cestrum yellow leaf curling virus (CYLCV)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
  • BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)
  • BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-104789-2 eCry3.1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera), Northern corn rootworm (Diabrotica barberi))
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
Southern blot analysis indicated that a single intact copy of the transformation cassette was inserted into the maize genome and that there was no integration of any backbone vector fragments. Nucleotide sequencing indicated that the the regulatory and functional elements of the insert were the same as those present in the pSYN12274 plasmid. Sequence analysis also revealed that some truncation occurred at the right border (RB) and left border (LB) ends of the T-DNA during the transformation process.
LMO characteristics
  • Food
  • Feed
Detection method(s)
Additional Information