MON-87411-9 - Maize modified for herbicide tolerance and insect resistance | BCH-LMO-SCBD-108881 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 30 Sep 2015
Living Modified Organism identity
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Maize modified for herbicide tolerance and insect resistance
MON 87411
MON-87411-9 maize was modified to contain a suppression cassette that expresses an inverted repeat sequence designed to match the sequence of western corn rootworm (WCR).

The expression of the suppression cassette results in the formation of a double-stranded RNA (dsRNA) transcript containing a 240 bp fragment of the WCR Snf7 gene (DvSnf7). Upon consumption, the plant-produced dsRNA in MON 87411 is recognized by the CRW’s RNA interference (RNAi) machinery resulting in down regulation of the targeted DvSnf7 gene leading to CRW mortality.

MON 87411 also contains a cry3Bb1 gene that produces a modified Bacillus thuringiensis (subsp. kumamotoensis) Cry3Bb1 protein to protect against CRW larval feeding.

In addition, MON 87411 contains the cp4 epsps gene from Agrobacterium sp. strain CP4 that encodes for the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein, which confers tolerance to glyphosate
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-108875-2 Snf7 coding sequence | Diabrotica virgifera virgifera (Western corn rootworm, DIAVI)
    Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera))
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-14993-5 Cry3Bb1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
  • BCH-GENE-SCBD-108876-1 pIIG gene promoter | Zea mays (Maize, Corn, MAIZE)
  • BCH-GENE-SCBD-100354-6 5' untranslated leader from chlorophyll a/b-binding protein | Triticum aestivum (Wheat)
    Leader sequence
  • BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)
  • BCH-GENE-SCBD-100356-6 Heat shock protein 17.3 terminator | Triticum aestivum (Wheat)
  • BCH-GENE-SCBD-108877-1 Alpha Tubulin Gene promoter | Oryza sativa (Rice, ORYSA)
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-108880-1 Alpha Tubulin Gene terminator | Oryza sativa (Rice, ORYSA)
The DvSnf7p sequence is the partial coding sequence of the Snf7 gene from Diabrotica virgifera virgifera encoding the SNF7 subunit of the ESCRT-III complex. The DvSnf7 suppression cassette contains two 240 bp DvSnf7p sequences in an inverted orientation. There is an intervening sequence of 150 nucleotides between the two DvSnf7p sequences. When the suppression cassette is transcribed, the RNA expressed forms a hairpin loop thereby allowing the formation of double stranded DvSnf7 RNA. The DvSnf7p sequences in the suppression cassette produce a 240 bp dsRNA that upon transcription triggers the RNAi mechanism.

Sequencing, PCR, and bioinformatic analysis indicated that a single, intact insert of the DvSnf7 suppression cassette and the cry3Bb1 and cp4 epsps expression cassettes were stably integrated into the maize genome. Analyses also indicated the absence of the plasmid backbone DNA in MON 87411 maize.
LMO characteristics
Detection method(s)
Additional Information