Potato modified for increased oxygen supply to tubers | BCH-LMO-SCBD-110680 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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BCH-LMO-SCBD-110680-1   |   PDF   |   Print   |  
Decisions on the LMO Risk Assessments  
last updated: 13 Jul 2016
Living Modified Organism identity
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Potato modified for increased oxygen supply to tubers
EN
B33-LegHg-3‘OCS 13; B33-LegHg-3‘OCS 45; B33-LegHg-3‘OCS 54 and B33-LegHg-3‘OCS 57
No
The potato plants were genetically modified to express the Leghemoglobin gene from [i[Lotus japonicus[/i] under the control of a tuber-specific promoter.

The genetic modification is expected to lead to increased oxygen content in the tuber at up to a depth of 2 mm below the skin, leading to enlarged lenticels and an increased tuber starch content.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar/breeding line: Desirée
EN
Characteristics of the modification process
Derivative of pART27 (B33-LegHg-3'OCS)
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100273-4 B33 gene promotor | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-110675-1 Leghaemoglobin gene | Lotus japonicus (Birdsfoot trefoil, LOTJA)
    Protein coding sequence | Changes in physiology and/or production,Changes in quality and/or metabolite content (Carbohydrates)
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
The papatin-B33 promoter from Solanum tuberosum mediates a tissue-specific expression of the leghaemoglobin gene in the tubers. The ocs terminator from Agrobacterium tumefaciens is used as a termination signal.

The nptII gene from Escherichia coli is expressed under the control of the promoter and terminator signal of the nopaline synthase gene from Agrobacterium tumefaciens and it was used as a selection marker.

The introduced nucleic acid is integrated in the genome of the recipient organism.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1