Potato modified for increased tuber weight | BCH-LMO-SCBD-111615 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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BCH-LMO-SCBD-111615-1   |   PDF   |   Print   |  
Decisions on the LMO Risk Assessments  
last updated: 07 Feb 2017
Living Modified Organism identity
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Potato modified for increased tuber weight
EN
B33-alphaUMPS 5, B33-alphaUMPS 8, B33-alphaUMPS 73, B33-alphaUMPS 76
No
The genetically modified potatoes were transformed to  constitutively express the cDNA coding of uridine monophosphate synthase (UMPS) from Solanum tuberosum in an antisense orientation. This results in the downregulation of endogenous UMPS which is expected to lead to a reduction in the de novo synthesis of uridine monophosphate (UMP).

The resulting altered UMP ratio indirectly influences starch biosynthesis. In wild-type plants, a portion of the cellular  glucose-1-phosphate is not used for starch biosynthesis but is converted to sucrose with the help of UDP-glucose-pyrophosphorylase (UGPase) and sucrose synthase. UGPase needs UTP for the synthesis of UDP-glucose. The reduced de novo synthesis of UMP would, by extension, lead to a reduced UTP content and therfore prevent the reconversion of glucose-1-phosphate to sucrose. More glucose-1-phosphate will, therefore, be available for starch biosynthesis, resulting in a 10-20% increase in tuber weight maintaining a constant density.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • Potato modified for increased tuber weight
    | Changes in physiology and/or production (Yield), Resistance to antibiotics (Kanamycin)
Characteristics of the modification process
Derivative of pBIN19
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100273-4 B33 gene promotor | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-111569-1 Uridine monophosphate synthase gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | Changes in physiology and/or production (Yield)
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Living Modified Organism Related LMO(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1