Potato modified for increased tuber weight | BCH-LMO-SCBD-111616 | Living Modified Organism | Biosafety Clearing-House

BCH-LMO-SCBD-111616-1   |   PDF   |   Print   |  

Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 07 Feb 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato modified for increased tuber weight
35S-alphaUMPS 21, 35S-alphaUMPS 26, 35S-alphaUMPS 29, 35S-alphaUMPS 73
The genetically modified potatoes were transformed to  constitutively express the cDNA coding of uridine monophosphate synthase (UMPS) from Solanum tuberosum in an antisense orientation. This results in the downregulation of endogenous UMPS which is expected to lead to a reduction in the de novo synthesis of uridine monophosphate (UMP).

The resulting altered UMP ratio indirectly influences starch biosynthesis. In wild-type plants, a portion of the cellular  glucose-1-phosphate is not used for starch biosynthesis but is converted to sucrose with the help of UDP-glucose-pyrophosphorylase (UGPase) and sucrose synthase. UGPase needs UTP for the synthesis of UDP-glucose. The reduced de novo synthesis of UMP would, by extension, lead to a reduced UTP content and therfore prevent the reconversion of glucose-1-phosphate to sucrose. More glucose-1-phosphate will, therefore, be available for starch biosynthesis, resulting in a 10-20% increase in tuber weight maintaining a constant density.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • Potato modified for increased tuber weight
    | Changes in physiology and/or production (Yield), Resistance to antibiotics (Kanamycin)
Characteristics of the modification process
Derivative of pBIN19
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-111569-1 Uridine monophosphate synthase gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | Changes in physiology and/or production (Yield)
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
LMO characteristics
  • Research
Detection method(s)
Additional Information
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