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Living Modified Organism (LMO)
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Insect resistant herbicide tolerant maizeEN
MZIR098 plants were modified to contain genes expressing ecry3.1Ab, mcry3A, and phosphinothricin acetyltransferase. These modification are intended to protect against certain coleopteran pests including western corn rootworm. Expression of the PAT gene, confers tolerance to glufosinate-ammonium herbicides and is used as a selection marker.EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Corn line: NP2222EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
BCH-GENE-SCBD-104789-2 eCry3.1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera), Northern corn rootworm (Diabrotica barberi))
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
BCH-GENE-SCBD-43634-3 mCry3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera))
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
BCH-GENE-SCBD-111966-2 Nopaline Synthase Enhancer | Agrobacterium tumefaciens (Agrobacterium)Leader
BCH-GENE-SCBD-104788-2 Cestrum Yellow Leaf Curling Virus promoter | Cestrum yellow leaf curling virus (CYLCV)Promoter
Molecular characterization of MZIR098 was carried out using nucleotide sequencing and Southern blot analyses. These analyses indicated that a single copy of the T-DNA was inserted in to the genome of the recipient organism and that and it does not contain the plasmid backbone sequence from transformation plasmid pSYN17629. Sequence analysis of the MZIR098 insertion site demonstrated that 24-bp from the corn genomic sequence was deleted during the integration of the MZIR098 insert.EN