CTC-14117-4 - Sugarcane modified for insect resistance | BCH-LMO-SCBD-112454 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 30 Aug 2017
Living Modified Organism identity
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Sugarcane modified for insect resistance
Sugarcane was modified with the insertion of gene cry1Ab to confer resistance to sugarcane borer, Diatraea saccharalis. The gene encoding neomycin phosphotransferase II, which confers resistance to aminoglycoside antibiotics such as kanamycin, was also integrated into the sugarcane as a selection marker.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: CTC20
Characteristics of the modification process
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-101404-3 Phosphoenolpyruvate Carboxylase gene promoter | Zea mays (Maize, Corn, MAIZE)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
Sugarcane is vegetatively propagated for commercial production. There is minimal likelihood of  transgene introgression via pollination.

CTB141175/01-A is a cultivar specific event. Southern blot analysis and sequence analysis indicated that multiple expression cassettes were integrated into the CTB141175/01-A event.

Southern blot analysis up to 6 generations indicated that all insertions were stable across several vegetative propagation cycles.

Western blot results confirmed the presence and integrity of Cry1Ab and NPTII proteins and absence of any other non-intended proteins.
LMO characteristics
  • Food
  • Feed
Detection method(s)
Additional Information
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