MON-887Ø2-4 - Insect-protected cotton | BCH-LMO-SCBD-114159 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 23 Jan 2023
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-protected cotton
Cotton (Gossypium hirsutum) modified to express the modified crystal protein mCry51Aa2 from Bacillus thuringiensis strain EG2934 to confer protection against Hemipteran and Thysanopteran insect pests, particularly tarnished plant bugs (Lygus hesperus and Lygus lineolaris), cotton fleahopper (Pseudoalomoscelis seriatus) and thrips ( Frankiniella spp.).
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar DP 393
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
0.410 kb
1.010 kb
0.920 kb
0.200 kb
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-114157-2 mCry51Aa2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects)
  • BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
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    BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
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    BCH-GENE-SCBD-114158-2 Heat shock protein 81-2 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
The modified cotton contains one gene cassette: Bacullis thuringiensis mCry51Aa2.

Transcription of mCry51Aa2 coding sequence directed by the Arabidopsis thaliana heat shock protein 81-2 promoter (P-hsp81-2), with enhanced activity due to the Figwort mosaic virus 35S enhancer (E-FMV) located adjacent to the promoter. Transcription is terminated by a Cauliflower mosaic virus (CaMV) 35S terminator (T-CaMV). Due to the enhancer, high levels of expression are expected.

  • Next-generation sequencing confirmed the vector backbone was not incorporated into the plant and the presence of a single T-DNA integration site.
  • The plasmid PV-GHIR508523 contained two T-DNA regions:
    1. E-FMV, P-hsp81-2, mCry51Aa2, and P-Hsp81-2; and
    2. E-FMV, P-EF-1α (Elongation Factor 1 alpha promoter), TS-CTP2 (N-terminal chloroplast peptide), aadA (Escherichia coli streptomycin 3''-adenylyltransferase), and T-E9 (Pisum sativum rbcS-E9 gene terminator).
    • The T-DNAII was crossed out due to selective breeding and was not present in the final cotton line.
  • MCry51Aa2 is a 302 amino acid, 33.6kDa single polypeptide. This modified protein contains eight amino acid substitutions(F46S, Y54H, S95A, F147S, Q149E, S167R, P219R, R273W) and a deletion of three amino acids (Δ196-198), which improve the effectiveness of the protein against the major Hemipteran cotton pests Lygus hesperus and Lygus lineolaris.  Immunoblotting of leaf samples and enzyme-linked immunosorbent assay (ELISA) of leaf, pollen, root, and seed samples confirmed the expression of the protein.

LMO characteristics
  • Feed
  • Food
Detection method(s)