AAT-7Ø9AA-4 - Pod Borer-resistant cowpea | BCH-LMO-SCBD-114444 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 26 Feb 2019
Living Modified Organism identity
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Pod Borer-resistant cowpea
Cowpea cultivar IT86D-1010 was modified via Agrobacterium-mediated transformation for the expression a truncated version of the Cry1Ab protein from Bacillus thuringiensis subs. kurstaki strain HD-1 for resistance against Pod Borer (Maruca vitrata).
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
pMB4 derived from pART27
  • Agrobacterium-mediated DNA transfer
1.730 kb
1.850 kb
0.370 kb
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Transcription of cry1Ab occurs from the Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit promoter, which also contains the 5' untranslated region. Transcription terminates at the Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator. The nucleotide sequence of cry1Ab was codon optimized for plant expression and encodes a 615-amino acid protein (68.9 kDa), corresponding to the trypsin-resistant, insecticidally active core protein, following cleavage of the 1155-amino acid native Cry1Ab protoxin.

For selection during cloning, a kanamycin resistance gene cassette was also integrated into the genome. Transcription of the neomycin phosphotranferase II (nphII) gene starts at the subterranean clover stunt virus (SCSV) 1 promoter. Two segments of nptII are transcribed, with a catalase-1 intron from Ricinus communis between the two segments and an intervening sequence from the MB4 plasmid following the second segment. Transcription stops at the SCSV3 terminator.
LMO characteristics
  • Food
Detection method(s)
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 2
Risk Assessment generated by a regulatory process Living modified organism(s) 2
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 1
Living Modified Organism Related LMO(s) 2