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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Pod Borer-resistant cowpea
EN
AAT709A
Yes
AAT-7Ø9AA-4
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Person:AATF and Institute of Agricultural ResearchARCN Annex No. 3 Ibrahim Idris Street, Jabi( AATF) Institute of Agricultural Research, Ahmadu Bello University, P.M.B 1044, Zaria, Nigeria.Abuja, F.C.T
, NigeriaPhone: 254-204223700, +234 8028373464,Fax: 254-204223701,Email: rsadamu@gmail.com,Website:Related OrganizationAfrican Agricultural Technology Foundation and Institute of Agricultural Research ()Academic or research instituteARCN Annex No. 3 Ibrahim Idris Street, Jabi( AATF) Institute of Agricultural Research, Ahmadu Bello University, P.M.B 1044, Zaria, Nigeria.Abuja, F.C.T
, NigeriaPhone: 254-204223700, +234 8028373464,Fax: 254-204223701,Email: rsadamu@gmail.com,Website:
Cowpea cultivar IT86D-1010 was modified via Agrobacterium-mediated transformation for the expression a truncated version of the Cry1Ab protein from Bacillus thuringiensis subs. kurstaki strain HD-1 for resistance against Pod Borer (Maruca vitrata).
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-103617-3 Organism Vigna unguiculata (Cowpea, Black eyed pea)Crops
EN
pMB4 derived from pART27
EN
- Agrobacterium-mediated DNA transfer
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0.520 kb
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0.180 kb
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0.190 kb
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0.620 kb
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0.180 kb
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0.140 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-114438-2 SCSV1 promoter | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-114274-2 Catalase 1 intron | Ricinus communis (Castor bean)Intron
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BCH-GENE-SCBD-114439-4 pMB4 intervening sequenceCloning plasmid
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BCH-GENE-SCBD-114440-1 SCSV3 terminator | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)Terminator
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BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-103853-2 rbcS Terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
Transcription of cry1Ab occurs from the Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit promoter, which also contains the 5' untranslated region. Transcription terminates at the Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator. The nucleotide sequence of cry1Ab was codon optimized for plant expression and encodes a 615-amino acid protein (68.9 kDa), corresponding to the trypsin-resistant, insecticidally active core protein, following cleavage of the 1155-amino acid native Cry1Ab protoxin.
For selection during cloning, a kanamycin resistance gene cassette was also integrated into the genome. Transcription of the neomycin phosphotranferase II (nphII) gene starts at the subterranean clover stunt virus (SCSV) 1 promoter. Two segments of nptII are transcribed, with a catalase-1 intron from Ricinus communis between the two segments and an intervening sequence from the MB4 plasmid following the second segment. Transcription stops at the SCSV3 terminator.
EN
For selection during cloning, a kanamycin resistance gene cassette was also integrated into the genome. Transcription of the neomycin phosphotranferase II (nphII) gene starts at the subterranean clover stunt virus (SCSV) 1 promoter. Two segments of nptII are transcribed, with a catalase-1 intron from Ricinus communis between the two segments and an intervening sequence from the MB4 plasmid following the second segment. Transcription stops at the SCSV3 terminator.
EN
- Food
EN
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