DBN-Ø9ØØ4-6 - Glyphosate and Glufosinate tolerant soybean | BCH-LMO-SCBD-115067 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 26 Jul 2019
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Glyphosate and Glufosinate tolerant soybean
EN
DBN4003
Yes
DBN-Ø9ØØ4-6
The soybean was modified to express Agrobacterium tumefaciens CP4 EPSPS and Streptomyces viridochromogenes PAT proteins for tolerance to glyphosate and glufosinate herbicides, respectively. The bacterial CP4 EPSPS functions similarly to the endogenous enzyme, but has a greatly reduced affinity for glyphosate. The PAT protein detoxifes L-phosphinothricin (glufosinate) via the acetylation of the primary amino group.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pDBN9003
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-115065-1 Elongation Factor 1 alpha promoter | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Promoter
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
CP4 EPSPS:
Transcription of the Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) commences from the Glycine max Elongation factor 1 alpha promoter and terminates at the Pisum sativum  ribulose-1,5-bisphosphate carboxylase, small subunit (rbcS E9) terminator. An Arabidopsis thaliana chloroplast peptide 2 segment was added to the 5' end of cp4 epsps to target the final protein product to the chloroplast. The soy EF1α promoter is expected to promote high levels of transcription.

PAT:
Streptomyces viridochromogenes Phosphinothricin N-acetyltransferase (pat) gene is under control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and terminator. Elevated levels of transcription of pat are expected due to the CaMV 35S promoter. The protein is expected to remain in the cytoplasm of the plant cells.
EN
LMO characteristics
EN
  • Food
  • Feed
  • Biofuel
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1

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