DAS-24236-5 × DAS-21Ø23-5 × SYN-IR1Ø2-7 × DAS-8191Ø-7 - Herbicide tolerant, insect resistant cotton | BCH-LMO-SCBD-115554 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 27 Apr 2020
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide tolerant, insect resistant cotton
EN
281-24-236 × 3006-210-23 × COT102 × 81910
DAS-24236-5 × DAS-21Ø23-5 × SYN-IR1Ø2-7 × DAS-8191Ø-7
  • - Person: Monsanto do Brasil Ltda | BCH-CON-BR-100874-3
    Person
    Monsanto do Brasil Ltda
    Geraldo Ubirajara Berger Avenida das Nações Unidas, 12901 - Torre Norte - 7º andar
    Sao Paulo, SP
    04578-000, Brazil
    Phone: +55(11) 3383-8356,
    Fax: +55(11) 3383-8102 ; +55 (61) 3245-2523,
    Website:
    Related Organization
    Monsanto do Brasil Ltda ()
    Private sector (business and industry)
    Geraldo Ubirajara Berger Avenida das Nações Unidas, 12901 - Torre Norte - 7º andar
    Sao Paulo, SP
    04578-000, Brazil
    Phone: +55(11) 3383-8356,
    Fax: +55(11) 3383-8102 ; +55 (61) 3245-2523,
    Website:
The modified cotton was the result of crossing modified parental lines to achieve multiple herbicide tolerance and insect resistance. For Lepidopteran resistance, the modified cotton expresses Bacillus thuringiensis Cry1Ac, Cry1F and Vegetative insecticidal protein 3A in the aerial parts of the plant. For herbicide tolerance, the modified cotton expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase for tolerance to glufosinate, as well as Delftia acidovorans aryloxyalkanoate dioxygenase for tolerance to 2,4-dichlorophenoxyacetic acid. Additionally, an Escherichia coli hygromycin B phosphotransferase cassette is also present for hygromycin selection during transformation and breeding.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)
    Crops
  • BCH-LMO-SCBD-14940-7 Living Modified Organism DAS-24236-5 - Insect-resistant cotton
    Dow AgroSciences | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-LMO-SCBD-14938-7 Living Modified Organism DAS-21Ø23-5 - Insect-resistant cotton
    Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-LMO-SCBD-14992-8 Living Modified Organism SYN-IR1Ø2-7 - VIPCOT™ Cotton
    Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
  • BCH-LMO-SCBD-108872-2 Living Modified Organism DAS-8191Ø-7 - Cotton modified for herbicide tolerance
    XXXX | Resistance to herbicides (Glufosinate), Tolerance to 2,4-Dichlorophenoxyacetic acid
EN
Characteristics of the modification process
pAGM281; pMYC3006; pCOT-1; pDAB4468
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from 281-24-236 vector pAGM281
The DNA insertion expresses Bacillus thuringiensis var. aizawai cry1F and  Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).

Transcription of pat is under control of a Zea mays ubiquitin promoter and an Agrobacterium tumefaciens open reading frame 25 (ORF25) terminator. The transcript is expected to initially contain a Z. mays ubiquitin intron 1 at the 5’ end. The intron enhances the expression of pat.

Transcription of cry1F is under control of a synthetic 4ocs∆Mas2' promoter and the same ORF25 terminator as the pat expression cassette. The cassette is in the reverse orientation to utilize the same terminator.

Note:
- Transcription is expected to occur at elevated and constitutive levels due to the promoters.
- The coding sequences of pat and cry1F have been optimized for expression in plants
- Cry1F is a chimeric, full-length δ-endotoxin comprised of the core toxin of Cry1F and nontoxic portions of B. thuringiensis Cry1Ca3 and Cry1Ab1 proteins, which form the C-terminal end of the protein and are removed during the activation of the Cry1F protein.

DNA insert from 3006-210-23 vector pMYC3006
The insertion expresses Bacillus thuringiensis cry1Ac and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).

Transcription of pat is under control of a synthetic 4ocs∆Mas2' promoter and an Agrobacterium tumefaciens open reading frame 25 (ORF25) terminator. Transcription of cry1Ac is under control of a Zea mays ubiquitin promoter and the same ORF25 terminator as the pat expression cassette.

Note:
- The ORF25 terminator serves as the terminator for both gene expression cassettes. The cry1Ac cassette is in the reverse orientation to utilize the same terminator.
- The coding sequences of cry1Ac and pat have been optimized for expression in plant cells.
- Transcription is expected to occur at elevated and constitutive levels due to the promoters.
- Southern blot analysis demonstrated that a single, intact DNA insertion was present in the parental genome. The analysis also indicated that no vector backbone sequences were integrated during transformation.

DNA insert from COT102 vector pCOT-1
The DNA insertion expresses Escherichia coli hygromycin B phosphotransferase (hph) and Bacillus thuringiensis vegetative insecticidal protein 3A.

Transcription of hph is under control of the Arabidopsis thaliana ubiquitin 3 promoter and the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The gene cassette is present in the counterclockwise orientation.

The expression of Bacillus thuringiensis vegetative insecticidal protein 3A (vip3A) is under transcriptional control of the A. thaliana actin 2 promoter and the nos terminator.

Note:
- The coding sequence of the vip3A was altered for optimal expression in plant cells.
- Transcription is expected to occur at elevated and constitutive levels due to the promoters.
- Southern blot analysis indicated the incorporation of a single copy of the transgenes without the integration of the vector backbone sequences.
- Southern blot analysis confirmed the expression of the proteins.

DNA insert from 81910 vector DAB4468
The DNA insertion expresses Delftia acidovorans aryloxyalkanoate dioxygenase (aad-12) and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (pat).

Transcription of aad-12 is under control of the Arabidopsis thaliana polyubiquitin 10 promoter and the Agrobacterium tumefaciens open reading frame 23 3’ untranslated region. Transcription of pat is under control of the Cassava Vein Mosaic Virus promoter and the A. tumefaciens ORF1 3’ untranslated region.

Note:
- Transcription is expected to occur at elevated and constitutive levels due to the promoters.
- Southern blot analysis indicated a single, intact insertion of aad-12 and pat into the parental genome without the integration of the vector backbone sequences.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
EN
Additional Information
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