CTC-932Ø9-4 - Insect resistant sugarcane | BCH-LMO-SCBD-115563 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 17 Feb 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant sugarcane
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CTC93209
CTC-932Ø9-4
  • - Organization: CTC - Centro de Tecnologia Canavieira (CTC) | BCH-CON-SCBD-243818-1
    Organization
    CTC - Centro de Tecnologia Canavieira (CTC)
    Regional economic integration organization
    Fazenda Santo Antônio s/n, Caixa Postal 162
    Piracicaba, Sao Paulo
    13400-970, Brazil
    Phone: +55 (11) 3429-8111,
    Fax: +55 (11) 3429-8111,
The sugarcane (Saccharum sp.) was modified to express Bacillus thuringiensis Cry1Ac with the aim of controlling the sugarcane borer (Diatraea saccharalis). The modified sugarcane also contains a selectable marker, Escherichia coli neomycin phosphotransferase II, for kanamycin selection during transformation.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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  • CTC-14117-4 - Sugarcane modified for insect resistance
    | Dr Wladecir Salles Oliveira Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Characteristics of the modification process
pCTC523
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The event CTC93209 was obtained using Agrobacterium-mediated transformation to insert T-DNA from the vector pCTC523. The inserted fragment contains two expression cassettes: Bacillus thuringiensis cry1Ac and Escherichia coli neomycin phosphotransferase II (nptII).

The cry1Ac coding sequence is under control of the Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator.

The nptII coding sequence is under control of the Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator.

Note:
  • Restriction digest analysis indicated that the sugarcane genome contains two T-DNA inserts.
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LMO characteristics
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  • Feed
  • Food
Detection method(s)
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Additional Information
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